An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora)
Publication type: Journal Article
Publication date: 1994-01-01
SJR: —
CiteScore: —
Impact factor: —
ISSN: 00221589
Plant Science
Abstract
SummaryThe standard method (protocol 1) for disinfecting shoot expiants used for initiation of plant tissue cultures failed when Iris rhizomes were infected with bacterial soft rot (Erwinia carotovora subsp, carotovora), Two new disinfection protocols were therefore developed, They involved keeping stock plants without irrigation for two months prior to excision of expiants; alcohol disinfection of the expiants prior to excision from the rhizome; dipping of the expiant in alcohol immediately after excision; soaking in 1 % (v/v) sodium hypochlorite (protocol 2) or saturated calcium hypochlorite (protocol 3); and soaking in mercuric chloride, With protocols 2 and 3 up to 90% and 62% respectively of expiants taken from infected Iris rhizomes showing soft-rot symptoms remained free of detectable microorganisms in vitro.
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Berger F., Waites W. M., Leifert C. An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora) // Journal of Horticultural Science. 1994. Vol. 69. No. 3. pp. 491-494.
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Berger F., Waites W. M., Leifert C. An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora) // Journal of Horticultural Science. 1994. Vol. 69. No. 3. pp. 491-494.
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TY - JOUR
DO - 10.1080/14620316.1994.11516480
UR - https://doi.org/10.1080/14620316.1994.11516480
TI - An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora)
T2 - Journal of Horticultural Science
AU - Berger, F
AU - Waites, W. M.
AU - Leifert, Carlo
PY - 1994
DA - 1994/01/01
PB - Taylor & Francis
SP - 491-494
IS - 3
VL - 69
SN - 0022-1589
ER -
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@article{1994_Berger,
author = {F Berger and W. M. Waites and Carlo Leifert},
title = {An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora)},
journal = {Journal of Horticultural Science},
year = {1994},
volume = {69},
publisher = {Taylor & Francis},
month = {jan},
url = {https://doi.org/10.1080/14620316.1994.11516480},
number = {3},
pages = {491--494},
doi = {10.1080/14620316.1994.11516480}
}
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MLA
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Berger, F., et al. “An improved surface disinfection method for shoot expiants fromIrisrhizomes infected with bacterial soft rot (Erwinia carotovorasubsp,carotovora).” Journal of Horticultural Science, vol. 69, no. 3, Jan. 1994, pp. 491-494. https://doi.org/10.1080/14620316.1994.11516480.