Open Access
Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells
Publication type: Journal Article
Publication date: 2021-06-01
SJR: 0.187
CiteScore: 1.3
Impact factor: —
ISSN: 17426588, 17426596
General Physics and Astronomy
Abstract
Exosomes and microvesicles usually require characterization at the single-particle level. The most commonly used methods for this purpose are transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA). However, atomic force microscopy (AFM) is also a promising method for the characterization of exosomes, and its opportunities seem underestimated. Here we describe the characterization of exosomes isolated from the conditioned media of human placenta cells using AFM, TEM, and NTA; we focus on the technical aspects of AFM imaging and data processing.
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Metrics
21
Total citations:
21
Citations from 2024:
12
(57.14%)
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MLA
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GOST
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Bagrov D. V. et al. Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells // Journal of Physics: Conference Series. 2021. Vol. 1942. No. 1. p. 12013.
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Bagrov D. V., Senkovenko A. M., Nikishin I. I., Skryabin G. O., Kopnin P., TCHEVKINA E. Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells // Journal of Physics: Conference Series. 2021. Vol. 1942. No. 1. p. 12013.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1088/1742-6596/1942/1/012013
UR - https://doi.org/10.1088/1742-6596/1942/1/012013
TI - Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells
T2 - Journal of Physics: Conference Series
AU - Bagrov, Dmitry V.
AU - Senkovenko, A M
AU - Nikishin, I I
AU - Skryabin, Gleb O
AU - Kopnin, Pavel
AU - TCHEVKINA, EM
PY - 2021
DA - 2021/06/01
PB - IOP Publishing
SP - 12013
IS - 1
VL - 1942
SN - 1742-6588
SN - 1742-6596
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2021_Bagrov,
author = {Dmitry V. Bagrov and A M Senkovenko and I I Nikishin and Gleb O Skryabin and Pavel Kopnin and EM TCHEVKINA},
title = {Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells},
journal = {Journal of Physics: Conference Series},
year = {2021},
volume = {1942},
publisher = {IOP Publishing},
month = {jun},
url = {https://doi.org/10.1088/1742-6596/1942/1/012013},
number = {1},
pages = {12013},
doi = {10.1088/1742-6596/1942/1/012013}
}
Cite this
MLA
Copy
Bagrov, Dmitry V., et al. “Application of AFM, TEM, and NTA for characterization of exosomes produced by placenta-derived mesenchymal cells.” Journal of Physics: Conference Series, vol. 1942, no. 1, Jun. 2021, p. 12013. https://doi.org/10.1088/1742-6596/1942/1/012013.