Open Access
3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function
Тип публикации: Journal Article
Дата публикации: 2016-04-27
scimago Q3
wos Q3
БС1
SJR: 0.376
CiteScore: 2.5
Impact factor: 1.6
ISSN: 20571976
General Nursing
Краткое описание
Zr-Si organic–inorganic scaffolds fabricated by a two-photon polymerization technique were used for the primary culture of mouse embryonic neural cells. We observed that dissociated hippocampal cells adhere to the scaffolds, produce neurites, elongate and differentiate into adult neurons. Neuronal outgrowth and synaptogenesis were confirmed by immunohistochemical staining with antibodies against βIII-Tubulin and synaptophysin. The formation of a functional neural network was assessed by the measurement of spontaneous activity using Ca2+ imaging of dissociated hippocampal cultures grown on Zr-Si scaffolds. The results of this study suggest that two-photon-induced polymerization of organic–inorganic hybrid biomaterials provides a robust model for 3D neuronal tissue engineering studies.
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ГОСТ
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Timashev P. S. et al. 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function // Biomedical Physics and Engineering Express. 2016. Vol. 2. No. 3. p. 35001.
ГОСТ со всеми авторами (до 50)
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Timashev P. S., Vedunova M. V., Guseva D., Ponimaskin E., Deiwick A., Mishchenko T., Mitroshina E. V., Koroleva A., Pimashkin A., Mukhina I., Panchenko V. Ya., Chichkov B. N., Bagratashvili V. N. 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function // Biomedical Physics and Engineering Express. 2016. Vol. 2. No. 3. p. 35001.
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RIS
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TY - JOUR
DO - 10.1088/2057-1976/2/3/035001
UR - https://doi.org/10.1088/2057-1976/2/3/035001
TI - 3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function
T2 - Biomedical Physics and Engineering Express
AU - Timashev, Petr S.
AU - Vedunova, M V
AU - Guseva, D.
AU - Ponimaskin, E.
AU - Deiwick, A.
AU - Mishchenko, Tatiana
AU - Mitroshina, E V
AU - Koroleva, Anastasia
AU - Pimashkin, Alexey
AU - Mukhina, I.V.
AU - Panchenko, V Ya
AU - Chichkov, Boris N.
AU - Bagratashvili, Victor N.
PY - 2016
DA - 2016/04/27
PB - IOP Publishing
SP - 35001
IS - 3
VL - 2
SN - 2057-1976
ER -
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BibTex (до 50 авторов)
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@article{2016_Timashev,
author = {Petr S. Timashev and M V Vedunova and D. Guseva and E. Ponimaskin and A. Deiwick and Tatiana Mishchenko and E V Mitroshina and Anastasia Koroleva and Alexey Pimashkin and I.V. Mukhina and V Ya Panchenko and Boris N. Chichkov and Victor N. Bagratashvili},
title = {3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function},
journal = {Biomedical Physics and Engineering Express},
year = {2016},
volume = {2},
publisher = {IOP Publishing},
month = {apr},
url = {https://doi.org/10.1088/2057-1976/2/3/035001},
number = {3},
pages = {35001},
doi = {10.1088/2057-1976/2/3/035001}
}
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MLA
Скопировать
Timashev, P. S., et al. “3D in vitro platform produced by two-photon polymerization for the analysis of neural network formation and function.” Biomedical Physics and Engineering Express, vol. 2, no. 3, Apr. 2016, p. 35001. https://doi.org/10.1088/2057-1976/2/3/035001.