Open Access
Molecular Biology of the Cell, volume 14, issue 1, pages 1-13
N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex
1
Vanderbilt University, Department of Biological Sciences, Nashville, Tennessee 37235;
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2
Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309-0215; and
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3
Department of Plant Sciences, Montana State University, Bozeman, Montana 59717
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Publication type: Journal Article
Publication date: 2003-01-16
Journal:
Molecular Biology of the Cell
Quartile SCImago
Q1
Quartile WOS
Q3
Impact factor: 3.3
ISSN: 10591524, 19394586
PubMed ID:
12529422
Molecular Biology
Cell Biology
Abstract
Telomerase is a reverse transcriptase that maintains chromosome ends. The N-terminal half of the catalytic protein subunit (TERT) contains three functional domains (I, II, and III) that are conserved among TERTs but not found in other reverse transcriptases. Guided by an amino acid sequence alignment of nine TERT proteins, mutations were introduced into yeast TERT (Est2p). In support of the proposed alignment, mutation of virtually all conserved residues resulted in loss-of-function or temperature sensitivity, accompanied by telomere shortening. Overexpression of telomerase component Est3p led to allele-specific suppression of the temperature-sensitive mutations in region I, suggesting that Est3p interacts with this protein domain. As predicted by the genetic results, a lethal mutation in region I resulted in loss of Est3p from the telomerase complex. We conclude that Est2p region I is required for the recruitment of Est3p to yeast telomerase. Given the phylogenetic conservation of region I of TERT, this protein domain may provide the equivalent function in all telomerases.
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Friedman K. L. et al. N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex // Molecular Biology of the Cell. 2003. Vol. 14. No. 1. pp. 1-13.
GOST all authors (up to 50)
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Friedman K. L., Heit J. J., LONG D. M., Cech T. R. N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex // Molecular Biology of the Cell. 2003. Vol. 14. No. 1. pp. 1-13.
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TY - JOUR
DO - 10.1091/mbc.E02-06-0327
UR - https://doi.org/10.1091%2Fmbc.E02-06-0327
TI - N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex
T2 - Molecular Biology of the Cell
AU - Friedman, Katherine L.
AU - Heit, Jeremy J.
AU - LONG, DAVID M.
AU - Cech, Thomas R
PY - 2003
DA - 2003/01/16 00:00:00
PB - American Society for Cell Biology (ASCB)
SP - 1-13
IS - 1
VL - 14
PMID - 12529422
SN - 1059-1524
SN - 1939-4586
ER -
Cite this
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@article{2003_Friedman,
author = {Katherine L. Friedman and Jeremy J. Heit and DAVID M. LONG and Thomas R Cech},
title = {N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex},
journal = {Molecular Biology of the Cell},
year = {2003},
volume = {14},
publisher = {American Society for Cell Biology (ASCB)},
month = {jan},
url = {https://doi.org/10.1091%2Fmbc.E02-06-0327},
number = {1},
pages = {1--13},
doi = {10.1091/mbc.E02-06-0327}
}
Cite this
MLA
Copy
Friedman, Katherine L., et al. “N-terminal Domain of Yeast Telomerase Reverse Transcriptase: Recruitment of Est3p to the Telomerase Complex.” Molecular Biology of the Cell, vol. 14, no. 1, Jan. 2003, pp. 1-13. https://doi.org/10.1091%2Fmbc.E02-06-0327.