Open Access
Annals of Botany, volume 110, issue 2, pages 479-489
Composite Cucurbita pepo plants with transgenic roots as a tool to study root development
Ilina Elena L
1, 2
,
Logachov Anton A
3
,
Laplaze Laurent
4
,
Demchenko Nikolay P
3
,
Pawlowski Katharina
5
,
1
Komarov Botanical Inst., Russian Academy of Sciences
|
2
St Petersburg Russia
|
4
Institut de Recherche pour le Développement, UMR DIADE (Agro.M/INRA/IRD/UM2), Equipe Rhizogenèse, 911 Avenue Agropolis, F-34394 Montpellier cedex 5, France
|
Publication type: Journal Article
Publication date: 2012-05-02
Journal:
Annals of Botany
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 4.2
ISSN: 03057364, 10958290
Plant Science
Abstract
In most plant species, initiation of lateral root primordia occurs above the elongation zone. However, in cucurbits and some other species, lateral root primordia initiation and development takes place in the apical meristem of the parental root. Composite transgenic plants obtained by Agrobacterium rhizogenes-mediated transformation are known as a suitable model to study root development. The aim of the present study was to establish this transformation technique for squash.The auxin-responsive promoter DR5 was cloned into the binary vectors pKGW-RR-MGW and pMDC162-GFP. Incorporation of 5-ethynyl-2'-deoxyuridine (EdU) was used to evaluate the presence of DNA-synthesizing cells in the hypocotyl of squash seedlings to find out whether they were suitable for infection. Two A. rhizogenes strains, R1000 and MSU440, were used. Roots containing the respective constructs were selected based on DsRED1 or green fluorescent protein (GFP) fluorescence, and DR5::Egfp-gusA or DR5::gusA insertion, respectively, was verified by PCR. Distribution of the response to auxin was visualized by GFP fluorescence or β-glucuronidase (GUS) activity staining and confirmed by immunolocalization of GFP and GUS proteins, respectively.Based on the distribution of EdU-labelled cells, it was determined that 6-day-old squash seedlings were suited for inoculation by A. rhizogenes since their root pericycle and the adjacent layers contain enough proliferating cells. Agrobacterium rhizogenes R1000 proved to be the most virulent strain on squash seedlings. Squash roots containing the respective constructs did not exhibit the hairy root phenotype and were morphologically and structurally similar to wild-type roots.The auxin response pattern in the root apex of squash resembled that in arabidopsis roots. Composite squash plants obtained by A. rhizogenes-mediated transformation are a good tool for the investigation of root apical meristem development and root branching.
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Ilina E. L. et al. Composite Cucurbita pepo plants with transgenic roots as a tool to study root development // Annals of Botany. 2012. Vol. 110. No. 2. pp. 479-489.
GOST all authors (up to 50)
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Ilina E. L., Logachov A. A., Laplaze L., Demchenko N. P., Pawlowski K., Demchenko K. Composite Cucurbita pepo plants with transgenic roots as a tool to study root development // Annals of Botany. 2012. Vol. 110. No. 2. pp. 479-489.
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RIS
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TY - JOUR
DO - 10.1093/aob/mcs086
UR - https://doi.org/10.1093%2Faob%2Fmcs086
TI - Composite Cucurbita pepo plants with transgenic roots as a tool to study root development
T2 - Annals of Botany
AU - Ilina, Elena L
AU - Logachov, Anton A
AU - Laplaze, Laurent
AU - Demchenko, Nikolay P
AU - Pawlowski, Katharina
AU - Demchenko, Kirill
PY - 2012
DA - 2012/05/02 00:00:00
PB - Oxford University Press
SP - 479-489
IS - 2
VL - 110
SN - 0305-7364
SN - 1095-8290
ER -
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@article{2012_Ilina,
author = {Elena L Ilina and Anton A Logachov and Laurent Laplaze and Nikolay P Demchenko and Katharina Pawlowski and Kirill Demchenko},
title = {Composite Cucurbita pepo plants with transgenic roots as a tool to study root development},
journal = {Annals of Botany},
year = {2012},
volume = {110},
publisher = {Oxford University Press},
month = {may},
url = {https://doi.org/10.1093%2Faob%2Fmcs086},
number = {2},
pages = {479--489},
doi = {10.1093/aob/mcs086}
}
Cite this
MLA
Copy
Ilina, Elena L., et al. “Composite Cucurbita pepo plants with transgenic roots as a tool to study root development.” Annals of Botany, vol. 110, no. 2, May. 2012, pp. 479-489. https://doi.org/10.1093%2Faob%2Fmcs086.
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