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Annals of Botany

Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player?

Ilina Elena L ¹

Kiryushkin Alexey S ¹

Semenova Victoria A ¹

Demchenko Nikolay P ¹

Pawlowski Katharina ²

Demchenko Kirill ^{1, 3}

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Laboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, Saint-Petersburg, Russia |

Department of Ecology, Environment and Plant Sciences, Stockholm university, Stockholm, Sweden |

Laboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Podbelsky chaussee, Pushkin, Saint-Petersburg, Russia |

Publication type: Journal Article

Publication date: 2018-04-19

Oxford University Press

Journal: Annals of Botany

Quartile SCImago

Quartile WOS

Impact factor: 4.2

ISSN: 03057364, 10958290

DOI: 10.1093/aob/mcy052

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Plant Science

Abstract

In some plant families, including Cucurbitaceae, initiation and development of lateral roots (LRs) occur in the parental root apical meristem. The objective of this study was to identify the general mechanisms underlying LR initiation (LRI). Therefore, the first cellular events leading to LRI as well as the role of auxin in this process were studied in the Cucurbita pepo root apical meristem.Transgenic hairy roots harbouring the auxin-responsive promoter DR5 fused to different reporter genes were used for visualizing of cellular auxin response maxima (ARMs) via confocal laser scanning microscopy and 3-D imaging. The effects of exogenous auxin and auxin transport inhibitors on root branching were analysed.The earliest LRI event involved a group of symmetric anticlinal divisions in pericycle cell files at a distance of 250-350 µm from the initial cells. The visualization of the ARMs enabled the precise detection of cells involved in determining the site of LR primordium formation. A local ARM appeared in sister cells of the pericycle and endodermis files before the first division. Cortical cells contributed to LR development after the anticlinal divisions in the pericycle via the formation of an ARM. Exogenous auxins did not increase the total number of LRs and did not affect the LRI index. Although exogenous auxin transport inhibitors acted in different ways, they all reduced the number of LRs formed.Literature data, as well as results obtained in this study, suggest that the formation of a local ARM before the first anticlinal formative divisions is the common mechanism underlying LRI in flowering plants. We propose that the mechanisms of the regulation of root branching are independent of the position of the LRI site relative to the parental root tip.

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Citations by journals

	1 2 3 4
Frontiers in Plant Science	Frontiers in Plant Science, 4, 44.44% Frontiers in Plant Science 4 publications, 44.44%
Plants	Plants, 3, 33.33% Plants 3 publications, 33.33%
In Vitro Cellular and Developmental Biology - Plant	In Vitro Cellular and Developmental Biology - Plant, 1, 11.11% In Vitro Cellular and Developmental Biology - Plant 1 publication, 11.11%
	1 2 3 4

Citations by publishers

	1 2 3 4
Frontiers Media S.A.	Frontiers Media S.A., 4, 44.44% Frontiers Media S.A. 4 publications, 44.44%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 3, 33.33% Multidisciplinary Digital Publishing Institute (MDPI) 3 publications, 33.33%
Springer Nature	Springer Nature, 1, 11.11% Springer Nature 1 publication, 11.11%
	1 2 3 4

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Ilina E. L. et al. Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player? // Annals of Botany. 2018.

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Ilina E. L., Kiryushkin A. S., Semenova V. A., Demchenko N. P., Pawlowski K., Demchenko K. Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player? // Annals of Botany. 2018.

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