Open Access
Open access
Oxford University Press
Nucleic Acids Research
,  volume 18 ,  issue 22 ,  pages 6633-6636

UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines

Publication type: Journal Article
Publication date: 1990-01-01
Oxford University Press
scimago Q1
wos Q1
SJR: 7.776
CiteScore: 31.7
Impact factor: 13.1
ISSN: 03051048, 13624962
Genetics
Abstract
The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography, proton resonance spectroscopy, infrared spectroscopy and UV spectroscopy. Its photolysis upon UV irradiation was studied. After incorporation of this dATP analog into DNA containing the tet operator sequence the investigation of the interactions between tet operator DNA and Tet repressor protein by UV photocross-linking becomes possible. Photocross-linking of protein to DNA was demonstrated by the reduced migration of the DNA in SDS polyacrylamide gel electrophoresis. Addition of the inducer tetracycline prior to UV irradiation significantly reduces the DNA-protein cross-linking rate. The long wave UV light applied here does not significantly alter the DNA or the protein under the photocross-linking conditions.
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GOST |
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GOST Copy
MEFFERT R. et al. UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines // Nucleic Acids Research. 1990. Vol. 18. No. 22. pp. 6633-6636.
GOST all authors (up to 50) Copy
MEFFERT R., Rathgeber G., Schafer H. J., Dose K. UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines // Nucleic Acids Research. 1990. Vol. 18. No. 22. pp. 6633-6636.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1093/nar/18.22.6633
UR - https://doi.org/10.1093/nar/18.22.6633
TI - UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines
T2 - Nucleic Acids Research
AU - MEFFERT, RAINER
AU - Rathgeber, Gabriele
AU - Schafer, Hans Jochen
AU - Dose, Klaus
PY - 1990
DA - 1990/01/01
PB - Oxford University Press
SP - 6633-6636
IS - 22
VL - 18
PMID - 2251123
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{1990_MEFFERT,
author = {RAINER MEFFERT and Gabriele Rathgeber and Hans Jochen Schafer and Klaus Dose},
title = {UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines},
journal = {Nucleic Acids Research},
year = {1990},
volume = {18},
publisher = {Oxford University Press},
month = {jan},
url = {https://doi.org/10.1093/nar/18.22.6633},
number = {22},
pages = {6633--6636},
doi = {10.1093/nar/18.22.6633}
}
MLA
Cite this
MLA Copy
MEFFERT, RAINER, et al. “UV-induced cross-linking of Tet repressor to DNA containingtetoperator sequences and 8-azidoadenines.” Nucleic Acids Research, vol. 18, no. 22, Jan. 1990, pp. 6633-6636. https://doi.org/10.1093/nar/18.22.6633.