Open Access
EMBO Journal, volume 20, issue 13, pages 3526-3534
Human telomerase contains two cooperating telomerase RNA molecules
Publication type: Journal Article
Publication date: 2001-07-02
Journal:
EMBO Journal
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 11.4
ISSN: 02614189, 14602075
PubMed ID:
11432839
General Biochemistry, Genetics and Molecular Biology
Molecular Biology
General Immunology and Microbiology
General Neuroscience
Abstract
Telomerase uses a short stretch of its intrinsic RNA molecule as template for telomere repeat synthesis. Reverse transcription of the RNA template is catalyzed by the telomerase reverse transcriptase (TERT) protein subunit. We demonstrate that human telomerase reconstituted from recombinant TERT and telomerase RNA runs as a dimer on a gel filtration column and that it contains two telomerase RNA molecules. Significantly, a telomerase heterodimer reconstituted from wild‐type and mutant telomerase RNA is barely active when compared with the wild‐type homodimer. We conclude that the telomerase RNA templates in the active enzyme are interdependent and functionally cooperate with each other. We discuss models that may explain the biological and enzymatic roles of telomerase dimerization.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Wenz C. Human telomerase contains two cooperating telomerase RNA molecules // EMBO Journal. 2001. Vol. 20. No. 13. pp. 3526-3534.
GOST all authors (up to 50)
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Wenz C. Human telomerase contains two cooperating telomerase RNA molecules // EMBO Journal. 2001. Vol. 20. No. 13. pp. 3526-3534.
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TY - JOUR
DO - 10.1093/emboj/20.13.3526
UR - https://doi.org/10.1093%2Femboj%2F20.13.3526
TI - Human telomerase contains two cooperating telomerase RNA molecules
T2 - EMBO Journal
AU - Wenz, C
PY - 2001
DA - 2001/07/02 00:00:00
PB - EMBO press
SP - 3526-3534
IS - 13
VL - 20
PMID - 11432839
SN - 0261-4189
SN - 1460-2075
ER -
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@article{2001_Wenz,
author = {C Wenz},
title = {Human telomerase contains two cooperating telomerase RNA molecules},
journal = {EMBO Journal},
year = {2001},
volume = {20},
publisher = {EMBO press},
month = {jul},
url = {https://doi.org/10.1093%2Femboj%2F20.13.3526},
number = {13},
pages = {3526--3534},
doi = {10.1093/emboj/20.13.3526}
}
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MLA
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Wenz, C.. “Human telomerase contains two cooperating telomerase RNA molecules.” EMBO Journal, vol. 20, no. 13, Jul. 2001, pp. 3526-3534. https://doi.org/10.1093%2Femboj%2F20.13.3526.