Open Access
Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA
Тип публикации: Journal Article
Дата публикации: 1987-01-01
scimago Q1
wos Q1
БС1
SJR: 7.048
CiteScore: 27.1
Impact factor: 13.1
ISSN: 03051048, 13624962
PubMed ID:
3588302
Genetics
Краткое описание
A series of dATP and dCTP nucleotide analogs have been synthesized which are modified by attachment of aliphatic linkers containing a functional group to the amino-nitrogen at the hydrogen bonding positions of the bases, that is, at the 6-position of adenine and the 4-position of cytosine. These nucleotides are incorporated into DNA probes by standard nick-translation protocols. DNA probes labeled with biotin derivatives of these nucleotides are effectively hybridized to target DNA sequences and can be detected by a streptavidin and calf intestinal alkaline phosphatase conjugate with a sensitivity (0.25 pg DNA) sufficient for reproducible and rapid detection of single copy genes in a Southern blot of mammalian DNA. Also, a procedure has been developed to allow reprobing of nylon filters that have been hybridized with biotinylated probes and developed with the streptavidin/alkaline phosphatase conjugate and a standard dye system.
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122
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122
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ГОСТ |
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MLA
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ГОСТ
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Gebeyehu G. et al. Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA // Nucleic Acids Research. 1987. Vol. 15. No. 11. pp. 4513-4534.
ГОСТ со всеми авторами (до 50)
Скопировать
Gebeyehu G., Rao P. Y., Soochan P., Simms D. A., Klevan L. Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA // Nucleic Acids Research. 1987. Vol. 15. No. 11. pp. 4513-4534.
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RIS
Скопировать
TY - JOUR
DO - 10.1093/nar/15.11.4513
UR - https://doi.org/10.1093/nar/15.11.4513
TI - Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA
T2 - Nucleic Acids Research
AU - Gebeyehu, Gulilat
AU - Rao, Prasad Y
AU - Soochan, Patricia
AU - Simms, D. A.
AU - Klevan, Leonard
PY - 1987
DA - 1987/01/01
PB - Oxford University Press
SP - 4513-4534
IS - 11
VL - 15
PMID - 3588302
SN - 0305-1048
SN - 1362-4962
ER -
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BibTex (до 50 авторов)
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@article{1987_Gebeyehu,
author = {Gulilat Gebeyehu and Prasad Y Rao and Patricia Soochan and D. A. Simms and Leonard Klevan},
title = {Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA},
journal = {Nucleic Acids Research},
year = {1987},
volume = {15},
publisher = {Oxford University Press},
month = {jan},
url = {https://doi.org/10.1093/nar/15.11.4513},
number = {11},
pages = {4513--4534},
doi = {10.1093/nar/15.11.4513}
}
Цитировать
MLA
Скопировать
Gebeyehu, Gulilat, et al. “Novel biotinylated nucleotide - analogs for labeling and colorimetric detection of DNA.” Nucleic Acids Research, vol. 15, no. 11, Jan. 1987, pp. 4513-4534. https://doi.org/10.1093/nar/15.11.4513.