Open Access
In situhybridisation with fluoresceinated DNA
Тип публикации: Journal Article
Дата публикации: 1991-01-01
scimago Q1
wos Q1
БС1
SJR: 7.048
CiteScore: 27.1
Impact factor: 13.1
ISSN: 03051048, 13624962
PubMed ID:
2062640
Genetics
Краткое описание
We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of microscopic imaging of in situ hybridized, fluoresceinated DNA with an integrating solid state camera for rapid cosmid mapping is illustrated. Combination of fluoresceinated DNA with biotinated and digoxigeninated DNAs allowed easy performance of triple fluorescence in situ hybridization. The potential of these techniques for DNA mapping, cytogenetics and biological dosimetry is briefly discussed.
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137
Всего цитирований:
137
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ГОСТ |
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MLA
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ГОСТ
Скопировать
Wiegant J. et al. In situhybridisation with fluoresceinated DNA // Nucleic Acids Research. 1991. Vol. 19. No. 12. pp. 3237-3241.
ГОСТ со всеми авторами (до 50)
Скопировать
Wiegant J., Ried T., Nederlof P. M., Ploeg M. V. D., Tanke H. J., Raap A. K. In situhybridisation with fluoresceinated DNA // Nucleic Acids Research. 1991. Vol. 19. No. 12. pp. 3237-3241.
Цитировать
RIS
Скопировать
TY - JOUR
DO - 10.1093/nar/19.12.3237
UR - https://doi.org/10.1093/nar/19.12.3237
TI - In situhybridisation with fluoresceinated DNA
T2 - Nucleic Acids Research
AU - Wiegant, Joop
AU - Ried, Thomas
AU - Nederlof, Petra M.
AU - Ploeg, Mels Van Der
AU - Tanke, Hans J.
AU - Raap, Anton K.
PY - 1991
DA - 1991/01/01
PB - Oxford University Press
SP - 3237-3241
IS - 12
VL - 19
PMID - 2062640
SN - 0305-1048
SN - 1362-4962
ER -
Цитировать
BibTex (до 50 авторов)
Скопировать
@article{1991_Wiegant,
author = {Joop Wiegant and Thomas Ried and Petra M. Nederlof and Mels Van Der Ploeg and Hans J. Tanke and Anton K. Raap},
title = {In situhybridisation with fluoresceinated DNA},
journal = {Nucleic Acids Research},
year = {1991},
volume = {19},
publisher = {Oxford University Press},
month = {jan},
url = {https://doi.org/10.1093/nar/19.12.3237},
number = {12},
pages = {3237--3241},
doi = {10.1093/nar/19.12.3237}
}
Цитировать
MLA
Скопировать
Wiegant, Joop, et al. “In situhybridisation with fluoresceinated DNA.” Nucleic Acids Research, vol. 19, no. 12, Jan. 1991, pp. 3237-3241. https://doi.org/10.1093/nar/19.12.3237.