Open Access
Nucleic Acids Research, volume 48, issue 6, pages 3366-3378
The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex
Publication type: Journal Article
Publication date: 2020-02-13
Journal:
Nucleic Acids Research
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 14.9
ISSN: 03051048, 13624962
Genetics
Abstract
RNAs play major roles in the regulation of gene expression. Hence, designer RNA molecules are increasingly explored as regulatory switches in synthetic biology. Among these, the TetR-binding RNA aptamer was selected by its ability to compete with operator DNA for binding to the bacterial repressor TetR. A fortuitous finding was that induction of TetR by tetracycline abolishes both RNA aptamer and operator DNA binding in TetR. This enabled numerous applications exploiting both the specificity of the RNA aptamer and the efficient gene repressor properties of TetR. Here, we present the crystal structure of the TetR-RNA aptamer complex at 2.7 Å resolution together with a comprehensive characterization of the TetR–RNA aptamer versus TetR–operator DNA interaction using site-directed mutagenesis, size exclusion chromatography, electrophoretic mobility shift assays and isothermal titration calorimetry. The fold of the RNA aptamer bears no resemblance to regular B-DNA, and neither does the thermodynamic characterization of the complex formation reaction. Nevertheless, the functional aptamer-binding epitope of TetR is fully contained within its DNA-binding epitope. In the RNA aptamer complex, TetR adopts the well-characterized DNA-binding-competent conformation of TetR, thus revealing how the synthetic TetR-binding aptamer strikes the chords of the bimodal allosteric behaviour of TetR to function as a synthetic regulator.
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Jaeger J. et al. The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex // Nucleic Acids Research. 2020. Vol. 48. No. 6. pp. 3366-3378.
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Jaeger J., Groher F., Muller Y. A., Grau F. C., Suess B. The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex // Nucleic Acids Research. 2020. Vol. 48. No. 6. pp. 3366-3378.
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TY - JOUR
DO - 10.1093/nar/gkaa083
UR - https://doi.org/10.1093/nar/gkaa083
TI - The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex
T2 - Nucleic Acids Research
AU - Jaeger, Jeannine
AU - Groher, Florian
AU - Muller, Yves A.
AU - Grau, Florian C.
AU - Suess, Beatrix
PY - 2020
DA - 2020/02/13 00:00:00
PB - Oxford University Press
SP - 3366-3378
IS - 6
VL - 48
SN - 0305-1048
SN - 1362-4962
ER -
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@article{2020_Jaeger,
author = {Jeannine Jaeger and Florian Groher and Yves A. Muller and Florian C. Grau and Beatrix Suess},
title = {The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex},
journal = {Nucleic Acids Research},
year = {2020},
volume = {48},
publisher = {Oxford University Press},
month = {feb},
url = {https://doi.org/10.1093/nar/gkaa083},
number = {6},
pages = {3366--3378},
doi = {10.1093/nar/gkaa083}
}
Cite this
MLA
Copy
Jaeger, Jeannine, et al. “The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex.” Nucleic Acids Research, vol. 48, no. 6, Feb. 2020, pp. 3366-3378. https://doi.org/10.1093/nar/gkaa083.