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Nucleic Acids Research, volume 50, issue 11, pages 6521-6531

Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila

Publication typeJournal Article
Publication date2022-06-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor14.9
ISSN03051048, 13624962
Genetics
Abstract

Transcriptional regulators select their targets from a large pool of similar genomic sites. The binding of the Drosophila dosage compensation complex (DCC) exclusively to the male X chromosome provides insight into binding site selectivity rules. Previous studies showed that the male-specific organizer of the complex, MSL2, and ubiquitous DNA-binding protein CLAMP directly interact and play an important role in the specificity of X chromosome binding. Here, we studied the highly specific interaction between the intrinsically disordered region of MSL2 and the N-terminal zinc-finger C2H2-type (C2H2) domain of CLAMP. We obtained the NMR structure of the CLAMP N-terminal C2H2 zinc finger, which has a classic C2H2 zinc-finger fold with a rather unusual distribution of residues typically used in DNA recognition. Substitutions of residues in this C2H2 domain had the same effect on the viability of males and females, suggesting that it plays a general role in CLAMP activity. The N-terminal C2H2 domain of CLAMP is highly conserved in insects. However, the MSL2 region involved in the interaction is conserved only within the Drosophila genus, suggesting that this interaction emerged during the evolution of a mechanism for the specific recruitment of the DCC on the male X chromosome in Drosophilidae.

Citations by journals

1
International Journal of Molecular Sciences
International Journal of Molecular Sciences, 1, 33.33%
International Journal of Molecular Sciences
1 publication, 33.33%
Nucleic Acids Research
Nucleic Acids Research, 1, 33.33%
Nucleic Acids Research
1 publication, 33.33%
1

Citations by publishers

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Multidisciplinary Digital Publishing Institute (MDPI)
Multidisciplinary Digital Publishing Institute (MDPI), 1, 33.33%
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 33.33%
Oxford University Press
Oxford University Press, 1, 33.33%
Oxford University Press
1 publication, 33.33%
1
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Tikhonova E. et al. Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila // Nucleic Acids Research. 2022. Vol. 50. No. 11. pp. 6521-6531.
GOST all authors (up to 50) Copy
Tikhonova E., Mariasina S., Efimov S., Polshakov V. I., Maksimenko O. G., Georgiev P. G., Бончук А. Н. Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila // Nucleic Acids Research. 2022. Vol. 50. No. 11. pp. 6521-6531.
RIS |
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TY - JOUR
DO - 10.1093/nar/gkac455
UR - https://doi.org/10.1093%2Fnar%2Fgkac455
TI - Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila
T2 - Nucleic Acids Research
AU - Tikhonova, Evgeniya
AU - Mariasina, Sofia
AU - Efimov, Sergey
AU - Polshakov, Vladimir I.
AU - Maksimenko, O. G.
AU - Georgiev, P. G.
AU - Бончук, А. Н.
PY - 2022
DA - 2022/06/01 00:00:00
PB - Oxford University Press
SP - 6521-6531
IS - 11
VL - 50
SN - 0305-1048
SN - 1362-4962
ER -
BibTex |
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BibTex Copy
@article{2022_Tikhonova
author = {Evgeniya Tikhonova and Sofia Mariasina and Sergey Efimov and Vladimir I. Polshakov and O. G. Maksimenko and P. G. Georgiev and А. Н. Бончук},
title = {Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila},
journal = {Nucleic Acids Research},
year = {2022},
volume = {50},
publisher = {Oxford University Press},
month = {jun},
url = {https://doi.org/10.1093%2Fnar%2Fgkac455},
number = {11},
pages = {6521--6531},
doi = {10.1093/nar/gkac455}
}
MLA
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Tikhonova, Evgeniya, et al. “Structural basis for interaction between CLAMP and MSL2 proteins involved in the specific recruitment of the dosage compensation complex in Drosophila.” Nucleic Acids Research, vol. 50, no. 11, Jun. 2022, pp. 6521-6531. https://doi.org/10.1093%2Fnar%2Fgkac455.
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