FASEB Journal, volume 35, issue 10

NOS‐dependent effects of plantar mechanical stimulation on mechanical characteristics and cytoskeletal proteins in rat soleus muscle during hindlimb suspension

Tyganov Sergey ¹

Mochalova Ekaterina P ¹

Melnikov Ivan ¹

Вихлянцев И. М. ²

Ulanova Anna D ²

Sharlo Kristina ¹

Mirzoev Timur M. ¹

Shenkman Boris ¹

Hide authors affiliations Show authors affiliations: 2 affiliations

Myology Laboratory Institute of Biomedical Problems Russian Academy of Sciences Moscow Russia |

Institute of Theoretical and Experimental Biophysics Russian Academy of Sciences Pushchino Russia |

Publication type: Journal Article

Publication date: 2021-09-27

Federation of American Societies for Experimental Biology (FASEB)

Journal: FASEB Journal

Quartile SCImago

Quartile WOS

Impact factor: 4.8

ISSN: 08926638, 15306860

DOI: 10.1096/fj.202100783R

Copy DOI

Biochemistry

Molecular Biology

Genetics

Biotechnology

Abstract

The study was aimed at investigating the mechanisms and structures which determine mechanical properties of skeletal muscles under gravitational unloading and plantar mechanical stimulation (PMS). We hypothesized that PMS would increase NO production and prevent an unloading‐induced reduction in skeletal muscle passive stiffness. Wistar rats were hindlimb suspended and subjected to a daily PMS and one group of stimulated animals was also treated with nitric oxide synthase (NOS) inhibitor (L‐NAME). Animals received mechanical stimulation of the feet for 4 h a day throughout 7‐day hindlimb suspension (HS) according to a scheme that mimics the normal walking of the animal. Seven‐day HS led to a significant reduction in soleus muscle weight by 25%. However, PMS did not prevent the atrophic effect induced by HS. Gravitational unloading led to a significant decrease in maximum isometric force and passive stiffness by 38% and 31%, respectively. The use of PMS prevented a decrease in the maximum isometric strength of the soleus muscle. At the same time, the passive stiffness of the soleus in the PMS group significantly exceeded the control values by 40%. L‐NAME (NOS inhibitor) administration attenuated the effect of PMS on passive stiffness and maximum force of the soleus muscle. The content of the studied cytoskeletal proteins (α‐actinin‐2, α‐actinin‐3, desmin, titin, nebulin) decreased after 7‐day HS, but this decrease was successfully prevented by PMS in a NOS‐dependent manner. We also observed significant decreases in mRNA expression levels of α‐actinin‐2, desmin, and titin after HS, which was prevented by PMS. The study also revealed a significant NOS‐dependent effect of PMS on the content of collagen‐1a, but not collagen‐3a. Thus, PMS during mechanical unloading is able to maintain soleus muscle passive tension and force as well as mRNA transcription and protein contents of cytoskeletal proteins in a NOS‐dependent manner.

By date By citations

Citations by journals

	1
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 25% International Journal of Molecular Sciences 1 publication, 25%
Pflugers Archiv European Journal of Physiology	Pflugers Archiv European Journal of Physiology, 1, 25% Pflugers Archiv European Journal of Physiology 1 publication, 25%
Biochemistry (Moscow)	Biochemistry (Moscow), 1, 25% Biochemistry (Moscow) 1 publication, 25%
Journal of Evolutionary Biochemistry and Physiology	Journal of Evolutionary Biochemistry and Physiology, 1, 25% Journal of Evolutionary Biochemistry and Physiology 1 publication, 25%
	1

Citations by publishers

	1 2
Pleiades Publishing	Pleiades Publishing, 2, 50% Pleiades Publishing 2 publications, 50%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 25% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 25%
Springer Nature	Springer Nature, 1, 25% Springer Nature 1 publication, 25%
	1 2

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[1,2,0,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["25","50",0,"25"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["International Journal of Molecular Sciences","Pflugers Archiv European Journal of Physiology","Biochemistry (Moscow)","Journal of Evolutionary Biochemistry and Physiology"],"ids":[14627,14088,2119,26727],"codes":[0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[25,25,25,25],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Pleiades Publishing","Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature"],"ids":[101,202,8],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[2,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[50,25,25],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Metrics

Cite this

GOST |

Cite this

GOST Copy

Tyganov S. et al. NOS‐dependent effects of plantar mechanical stimulation on mechanical characteristics and cytoskeletal proteins in rat soleus muscle during hindlimb suspension // FASEB Journal. 2021. Vol. 35. No. 10.

GOST all authors (up to 50) Copy

Tyganov S., Mochalova E. P., Melnikov I., Вихлянцев И. М., Ulanova A. D., Sharlo K., Mirzoev T. M., Shenkman B. NOS‐dependent effects of plantar mechanical stimulation on mechanical characteristics and cytoskeletal proteins in rat soleus muscle during hindlimb suspension // FASEB Journal. 2021. Vol. 35. No. 10.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1096/fj.202100783R

UR - https://doi.org/10.1096%2Ffj.202100783R

TI - NOS‐dependent effects of plantar mechanical stimulation on mechanical characteristics and cytoskeletal proteins in rat soleus muscle during hindlimb suspension

T2 - FASEB Journal

AU - Ulanova, Anna D

AU - Mochalova, Ekaterina P

AU - Tyganov, Sergey

AU - Melnikov, Ivan

AU - Вихлянцев, И. М.

AU - Sharlo, Kristina

AU - Mirzoev, Timur M.

AU - Shenkman, Boris

PY - 2021

DA - 2021/09/27 00:00:00

PB - Federation of American Societies for Experimental Biology (FASEB)

IS - 10

VL - 35

SN - 0892-6638

SN - 1530-6860

ER -

BibTex

Cite this

BibTex Copy

@article{2021_Tyganov,

author = {Anna D Ulanova and Ekaterina P Mochalova and Sergey Tyganov and Ivan Melnikov and И. М. Вихлянцев and Kristina Sharlo and Timur M. Mirzoev and Boris Shenkman},

title = {NOS‐dependent effects of plantar mechanical stimulation on mechanical characteristics and cytoskeletal proteins in rat soleus muscle during hindlimb suspension},

journal = {FASEB Journal},

year = {2021},

volume = {35},

publisher = {Federation of American Societies for Experimental Biology (FASEB)},

month = {sep},

url = {https://doi.org/10.1096%2Ffj.202100783R},

number = {10},

doi = {10.1096/fj.202100783R}

}

Found error?

Publisher

Federation of American Societies for Experimental Biology (FASEB)

Journal

FASEB Journal

Quartile SCImago

Quartile WOS

Impact factor

4.8

ISSN

08926638 (Print)
15306860 (Electronic)

Profiles

Tyganov, Sergey A

News

Ученые придумали, как защитить от атрофии мышцы космонавтов (и не только)