Sequential oxidation of L-lysine by a non-heme iron hydroxylase

2-oxoglutarate-dependent non-heme iron hydroxylases offer a direct route to functionalizing C(sp³)–H bonds across a diverse range of substrates, making them prime candidates for chemoenzymatic synthetic strategies. We demonstrate the ability of a non-heme iron L-lysine dioxygenase to perform sequential oxidation and computationally explore structural elements that promote this reactivity.