Open Access
Protein Science, volume 15, issue 2, pages 242-247
Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family
Publication type: Journal Article
Publication date: 2006-02-01
Journal:
Protein Science
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 8
ISSN: 09618368, 1469896X
PubMed ID:
16385000
Biochemistry
Molecular Biology
Abstract
Molecular chaperones of the Hsp70 family (bacterial DnaK, DnaJ, and GrpE) were shown to be strictly required for refolding of firefly luciferase from a denatured state and thus for effective restoration of its activity. At the same time the luciferase was found to be synthesized in an Escherichia coli cell‐free translation system in a highly active state in the extract with no chaperone activity. The addition of the chaperones to the extract during translation did not raise the activity of the enzyme. The abrupt arrest of translation by the addition of a translational inhibitor led to immediate cessation of the enzyme activity accumulation, indicating the cotranslational character of luciferase folding. The results presented suggest that the chaperones of the Hsp70 family are not required for effective cotranslational folding of firefly luciferase.
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Citations by publishers
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2
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12
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4 publications, 8.7%
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Wiley
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1 publication, 2.17%
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1 publication, 2.17%
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2
4
6
8
10
12
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Svetlov M. S. Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family // Protein Science. 2006. Vol. 15. No. 2. pp. 242-247.
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Svetlov M. S. Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family // Protein Science. 2006. Vol. 15. No. 2. pp. 242-247.
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TY - JOUR
DO - 10.1110/ps.051752506
UR - https://doi.org/10.1110%2Fps.051752506
TI - Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family
T2 - Protein Science
AU - Svetlov, M S
PY - 2006
DA - 2006/02/01 00:00:00
PB - Wiley
SP - 242-247
IS - 2
VL - 15
PMID - 16385000
SN - 0961-8368
SN - 1469-896X
ER -
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@article{2006_Svetlov,
author = {M S Svetlov},
title = {Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family},
journal = {Protein Science},
year = {2006},
volume = {15},
publisher = {Wiley},
month = {feb},
url = {https://doi.org/10.1110%2Fps.051752506},
number = {2},
pages = {242--247},
doi = {10.1110/ps.051752506}
}
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MLA
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Svetlov, M. S.. “Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family.” Protein Science, vol. 15, no. 2, Feb. 2006, pp. 242-247. https://doi.org/10.1110%2Fps.051752506.