Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa
1
Tokyo Research Laboratories; Kowa Co. Ltd.; Higashimurayama Tokyo
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Publication type: Journal Article
Publication date: 1972-01-01
PubMed ID:
4114204
General Medicine
Abstract
The distribution of the dihydrostreptomycin (DHSM)-phosphorylating enzyme was investigated using DHSM-resistant strains of Pseudomonas aeruginosa, indicating that this enzyme was demonstrated from all of 7 DHSM-resistant strains examined but not from a DHSM-sensitive one. The DHSM-phosphorylating enzyme was isolated from P. aeruginosa TI-13 and purified about 205-fold using Sephadex G-75 and DEAE-Sephadex A-50 column chromatography. The optimal pH for the DHSM-inactivation was around 10.0, and both adenosinetriphosphate (ATP) and Mg++ were required for the inactivating reaction. It was found that this enzyme inactivated only DHSM but not other aminoglycosidic antibiotics such as kanamycin, aminodeoxykanamycin, neomycin, paromomycin, lividomycin and gentamicin.
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Kobayashi F. et al. Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa // Japanese Journal of Microbiology. 1972. Vol. 16. No. 1. pp. 15-19.
GOST all authors (up to 50)
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Kobayashi F., YAMAGUCHI M., Sato J., Mitsuhashi S. Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa // Japanese Journal of Microbiology. 1972. Vol. 16. No. 1. pp. 15-19.
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RIS
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TY - JOUR
DO - 10.1111/j.1348-0421.1972.tb00622.x
UR - https://doi.org/10.1111/j.1348-0421.1972.tb00622.x
TI - Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa
T2 - Japanese Journal of Microbiology
AU - Kobayashi, Fujio
AU - YAMAGUCHI, Masahito
AU - Sato, Junko
AU - Mitsuhashi, Susumu
PY - 1972
DA - 1972/01/01
PB - Wiley
SP - 15-19
IS - 1
VL - 16
PMID - 4114204
SN - 0021-5139
ER -
Cite this
BibTex (up to 50 authors)
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@article{1972_Kobayashi,
author = {Fujio Kobayashi and Masahito YAMAGUCHI and Junko Sato and Susumu Mitsuhashi},
title = {Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa},
journal = {Japanese Journal of Microbiology},
year = {1972},
volume = {16},
publisher = {Wiley},
month = {jan},
url = {https://doi.org/10.1111/j.1348-0421.1972.tb00622.x},
number = {1},
pages = {15--19},
doi = {10.1111/j.1348-0421.1972.tb00622.x}
}
Cite this
MLA
Copy
Kobayashi, Fujio, et al. “Purification and Properties of Dihydrostreptomyein-Phosphorylating Enzyme fromPseudomonas aeruginosa.” Japanese Journal of Microbiology, vol. 16, no. 1, Jan. 1972, pp. 15-19. https://doi.org/10.1111/j.1348-0421.1972.tb00622.x.