Comparing conformations at low temperature and at high viscosity
Publication type: Journal Article
Publication date: 2009-01-12
SJR: —
CiteScore: —
Impact factor: —
ISSN: 03678377
Biochemistry
Abstract
The influence of low temperature and high viscosity on the conformation of somatostatin and two of its analogues was investigated by 1H NMR in solution. The conformation of native somatostatin, a cyclic octapeptide agonist DC13-116 and a linear octapeptide agonist were compared in ethylene glycol at 303 K and in methanol at low temperature. The first goal of this study was to investigate if either low temperature or high viscosity is the more important for the reduction of the conformational freedom. Secondly we wanted to compare the amount of information concerning the conformation present in both solvents. A larger amount of NOESY cross-peaks is observed in ethylene glycol at room temperature compared to methanol at low temperature. This indicates that the raising of the viscosity is a more important factor in reducing the flexibility of peptides than the lowering of the temperature.
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Total citations:
14
Citations from 2024:
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GOST
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Verheyden P. et al. Comparing conformations at low temperature and at high viscosity // International journal of peptide & protein research. 2009. Vol. 44. No. 5. pp. 401-409.
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Verheyden P., Wolf E., Jaspers H., Binst G. Comparing conformations at low temperature and at high viscosity // International journal of peptide & protein research. 2009. Vol. 44. No. 5. pp. 401-409.
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TY - JOUR
DO - 10.1111/j.1399-3011.1994.tb00174.x
UR - https://doi.org/10.1111/j.1399-3011.1994.tb00174.x
TI - Comparing conformations at low temperature and at high viscosity
T2 - International journal of peptide & protein research
AU - Verheyden, Patricia
AU - Wolf, Elke
AU - Jaspers, Hendrika
AU - Binst, Georges
PY - 2009
DA - 2009/01/12
PB - Wiley
SP - 401-409
IS - 5
VL - 44
SN - 0367-8377
ER -
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BibTex (up to 50 authors)
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@article{2009_Verheyden,
author = {Patricia Verheyden and Elke Wolf and Hendrika Jaspers and Georges Binst},
title = {Comparing conformations at low temperature and at high viscosity},
journal = {International journal of peptide & protein research},
year = {2009},
volume = {44},
publisher = {Wiley},
month = {jan},
url = {https://doi.org/10.1111/j.1399-3011.1994.tb00174.x},
number = {5},
pages = {401--409},
doi = {10.1111/j.1399-3011.1994.tb00174.x}
}
Cite this
MLA
Copy
Verheyden, Patricia, et al. “Comparing conformations at low temperature and at high viscosity.” International journal of peptide & protein research, vol. 44, no. 5, Jan. 2009, pp. 401-409. https://doi.org/10.1111/j.1399-3011.1994.tb00174.x.