International Endodontic Journal, volume 43, issue 5, pages 430-435

Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels

Tsung-Hsien Lee 1
C C Hu 2
S S Lee 3
M.Y. Chou 4, 5, 6
Y CHANG 7
1
 
Institute of Medicine, Chung Shan Medical University , Taichung, Taiwan.
2
 
School of Applied Chemistry, Chung Shan Medical University, Taichung
3
 
School of Public Health, Chung Shan Medical University, Taichung.
4
 
School of Dentistry
5
 
Chung-Shan Medical University
6
 
Taichung Taiwan
7
 
School of Dentistry, Chung Shan Medical University, Taichung, Taiwan
Publication typeJournal Article
Publication date2010-05-01
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor5
ISSN01432885, 13652591
General Dentistry
Abstract
To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro.Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH.CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of CHX was approximately 0.005%. CHX also inhibited cell proliferation and collagen synthesis (P < 0.05). The addition of OTZ acted as a protective effect on the CHX-induced cytotoxicity (P < 0.05). In contrast, the addition of BSO enhanced the CHX-induced cytotoxicity (P < 0.05).The levels of CHX tested inhibited cell growth, proliferation and collagen synthesis on U2OS cells. CHX has significant potential for periapical toxicity. GSH depletion might be one of the mechanisms underlying CHX cytotoxicity.

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GOST |
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GOST Copy
Lee T. et al. Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels // International Endodontic Journal. 2010. Vol. 43. No. 5. pp. 430-435.
GOST all authors (up to 50) Copy
Lee T., Hu C. C., Lee S. S., Chou M., CHANG Y. Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels // International Endodontic Journal. 2010. Vol. 43. No. 5. pp. 430-435.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1111/j.1365-2591.2010.01700.x
UR - https://doi.org/10.1111/j.1365-2591.2010.01700.x
TI - Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels
T2 - International Endodontic Journal
AU - Lee, Tsung-Hsien
AU - Hu, C C
AU - Lee, S S
AU - Chou, M.Y.
AU - CHANG, Y
PY - 2010
DA - 2010/05/01 00:00:00
PB - Wiley
SP - 430-435
IS - 5
VL - 43
SN - 0143-2885
SN - 1365-2591
ER -
BibTex |
Cite this
BibTex Copy
@article{2010_Lee,
author = {Tsung-Hsien Lee and C C Hu and S S Lee and M.Y. Chou and Y CHANG},
title = {Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels},
journal = {International Endodontic Journal},
year = {2010},
volume = {43},
publisher = {Wiley},
month = {may},
url = {https://doi.org/10.1111/j.1365-2591.2010.01700.x},
number = {5},
pages = {430--435},
doi = {10.1111/j.1365-2591.2010.01700.x}
}
MLA
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MLA Copy
Lee, Tsung-Hsien, et al. “Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels.” International Endodontic Journal, vol. 43, no. 5, May. 2010, pp. 430-435. https://doi.org/10.1111/j.1365-2591.2010.01700.x.
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