Two-photon excited polarized fluorescence in NADH in methanol/water solutions

Publication type: Proceedings Article
Publication date: 2019-07-22
SPIE-Intl Soc Optical Eng
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Abstract
We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions upon two-photon excitation with femtosecond laser pulses at 720 nm. The polarized fluorescence decay was recorded by a time correlated single photon counting (TCSPC) system. Fluorescence decay times, rotational diffusion time, fluorescence anisotropy, and the ratio of two pre-exponential factors have been determined and studied as a function of methanol concentration. The results obtained were interpreted on the basis of a model of NADH denaturation processes in solutions and can be used for modeling of NADH binding with various dehydrogenases in living cells.
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Gorbunova I. et al. Two-photon excited polarized fluorescence in NADH in methanol/water solutions // Advances in Microscopic Imaging II. 2019.
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Gorbunova I., Bezverkhnii N., Zhikhoreva A. A., Beltukov Y., Sasin M., Rubayo Soneira J., Vasyutinskii O. S. Two-photon excited polarized fluorescence in NADH in methanol/water solutions // Advances in Microscopic Imaging II. 2019.
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TY - CPAPER
DO - 10.1117/12.2536641
UR - https://doi.org/10.1117/12.2536641
TI - Two-photon excited polarized fluorescence in NADH in methanol/water solutions
T2 - Advances in Microscopic Imaging II
AU - Gorbunova, Ioanna
AU - Bezverkhnii, Nikolai
AU - Zhikhoreva, Anna A
AU - Beltukov, Yaroslav
AU - Sasin, Maxim
AU - Rubayo Soneira, Jesus
AU - Vasyutinskii, Oleg S.
PY - 2019
DA - 2019/07/22
PB - SPIE-Intl Soc Optical Eng
ER -
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@inproceedings{2019_Gorbunova,
author = {Ioanna Gorbunova and Nikolai Bezverkhnii and Anna A Zhikhoreva and Yaroslav Beltukov and Maxim Sasin and Jesus Rubayo Soneira and Oleg S. Vasyutinskii},
title = {Two-photon excited polarized fluorescence in NADH in methanol/water solutions},
year = {2019},
month = {jul},
publisher = {SPIE-Intl Soc Optical Eng}
}