Open Access

Science

, volume 340 , issue 6140 , pages 1580-1583

Transcription Under Torsion

Jie Ma ^{1, 2}

Lu Bai ^{3, 4}

Michelle D Wang ^{1, 2}

Hide authors affiliations Show authors affiliations: 4 affiliations

Department of Physics–Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, NY 14853, USA. |

Howard Hughes Medical Institute, Cornell University, Ithaca, NY 14853, USA.

Cornell University

Howard Hughes Medical Institute

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16801, USA. |

⁴

Department of Physics, Pennsylvania State University, University Park, PA 16801, USA. |

Publication type: Journal Article

Publication date: 2013-06-28

American Association for the Advancement of Science (AAAS)

Science

scimago Q1

wos Q1

SJR: 10.416

CiteScore: 48.4

Impact factor: 45.8

ISSN: 00368075, 10959203

DOI: 10.1126/science.1235441

Copy DOI

PubMed ID: 23812716

Multidisciplinary

Abstract

Keeping Transcription Going

In cells, the DNA double-stranded helix (dsDNA) is mostly supercoiled—either under- or overwound. RNA polymerase (RNAP) must transcribe though this supercoiled DNA. Furthermore, the act of transcription, which involves opening the double helix and threading the separated strands through the enzyme, generates supercoiling ahead and behind the polymerase. Ma et al. (p. 1580 ) used single-molecule methods to measure the upstream and downstream torque forces of Escherichia coli RNAP. The upstream torque was sufficient to disrupt dsDNA structure, and the stalled RNAP could also backtrack along the DNA. Release of the torsional stress allowed RNAP to resume transcription in vitro.

Found

1 citation

Bukatin Anton

86 publications, 986 citations, 15 reviews

h-index: 16

Institute for Analytical Instrumentation of Russian Academy of Sciences

Research interests

Microfluidics

1 citation

Ankudinov Alexander

95 publications, 589 citations

h-index: 13

Ioffe Physical-Technical Institute of the Russian Academy of Sciences

1 citation

Nieto-Vesperinas Manuel

237 publications, 9 460 citations

h-index: 48

Materials Science Institute of Madrid

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