Open Access

Science

, volume 370 , issue 6522

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

Jesse V. Veenvliet ¹

Adriano Bolondi ²

Helene Kretzmer ²

Leah Haut ^{1, 2}

Manuela Scholze-Wittler ¹

Dennis Schifferl ¹

Frederic Koch ¹

Léo Guignard ³

Abhishek Sampath Kumar ²

Milena Pustet ¹

Simon Heimann ¹

René Buschow ⁴

Lars Wittler ¹

Bernd Timmermann ⁵

Alexander Meissner ^{2, 6, 7, 8}

Bernhard G Herrmann ^{1, 9}

Hide authors affiliations Show authors affiliations: 9 affiliations

Department of Developmental Genetics, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany. |

Department of Genome Regulation, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany. |

Max Delbrück Center for Molecular Medicine and Berlin Institute of Health, 10115 Berlin, Germany. |

⁴

Microscopy and Cryo-Electron Microscopy, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany. |

⁵

Sequencing Core Facility, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany. |

⁶

Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA. |

⁷

Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. |

⁸

Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany. |

⁹

Institute for Medical Genetics, Charité–University Medicine Berlin, Campus Benjamin Franklin, 12203 Berlin, Germany. |

Publication type: Journal Article

Publication date: 2020-12-11

American Association for the Advancement of Science (AAAS)

Science

scimago Q1

wos Q1

SJR: 10.416

CiteScore: 48.4

Impact factor: 45.8

ISSN: 00368075, 10959203

DOI: 10.1126/science.aba4937

Copy DOI

PubMed ID: 33303587

Multidisciplinary

Abstract

Trunk formation in a dish

Building mammalian embryos from self-organizing stem cells in culture would accelerate the investigation of morphogenetic and differentiation processes that shape the body plan. Veenvliet et al. report a method for generating embryonic trunk-like structures (TLSs) with a neural tube, somites, and gut by embedding mouse embryonic stem cell aggregates in an extracellular matrix surrogate. Live imaging and comparative single-cell transcriptomics indicate that TLS formation is analogous to mouse development. TLSs therefore provide a scalable, tractable, and accessible high-throughput platform for decoding mammalian embryogenesis at a high level of resolution.

Science , this issue p. eaba4937

Found

1 citation

Novosad Yury

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10 publications, 7 citations

h-index: 2

Peter the Great St. Petersburg Polytechnic University

The Turner Scientific Research Institute for Children's Orthopedics

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Veenvliet J. V. et al. Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites // Science. 2020. Vol. 370. No. 6522.

GOST all authors (up to 50) Copy

Veenvliet J. V., Bolondi A., Kretzmer H., Haut L., Scholze-Wittler M., Schifferl D., Koch F., Guignard L., Kumar A. S., Pustet M., Heimann S., Buschow R., Wittler L., Timmermann B., Meissner A., Herrmann B. G. Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites // Science. 2020. Vol. 370. No. 6522.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1126/science.aba4937

UR - https://doi.org/10.1126/science.aba4937

TI - Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

T2 - Science

AU - Veenvliet, Jesse V.

AU - Bolondi, Adriano

AU - Kretzmer, Helene

AU - Haut, Leah

AU - Scholze-Wittler, Manuela

AU - Schifferl, Dennis

AU - Koch, Frederic

AU - Guignard, Léo

AU - Kumar, Abhishek Sampath

AU - Pustet, Milena

AU - Heimann, Simon

AU - Buschow, René

AU - Wittler, Lars

AU - Timmermann, Bernd

AU - Meissner, Alexander

AU - Herrmann, Bernhard G

PY - 2020

DA - 2020/12/11

PB - American Association for the Advancement of Science (AAAS)

IS - 6522

VL - 370

PMID - 33303587

SN - 0036-8075

SN - 1095-9203

ER -

BibTex

Cite this

BibTex (up to 50 authors) Copy

@article{2020_Veenvliet,

author = {Jesse V. Veenvliet and Adriano Bolondi and Helene Kretzmer and Leah Haut and Manuela Scholze-Wittler and Dennis Schifferl and Frederic Koch and Léo Guignard and Abhishek Sampath Kumar and Milena Pustet and Simon Heimann and René Buschow and Lars Wittler and Bernd Timmermann and Alexander Meissner and Bernhard G Herrmann},

title = {Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites},

journal = {Science},

year = {2020},

volume = {370},

publisher = {American Association for the Advancement of Science (AAAS)},

month = {dec},

url = {https://doi.org/10.1126/science.aba4937},

number = {6522},

doi = {10.1126/science.aba4937}

}

Publisher

American Association for the Advancement of Science (AAAS)

Journal

Science

scimago Q1

wos Q1

SJR

10.416

CiteScore

48.4

Impact factor

45.8

ISSN

00368075 (Print)

10959203 (Electronic)