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Science

, volume 317 , issue 5839 , pages 783-787

Label-Free, Single-Molecule Detection with Optical Microcavities

Andrea M Armani ^{1, 2, 3}

Rajan P Kulkarni ^{1, 2, 3}

Scott E. Fraser ^{1, 2, 3}

Richard C. Flagan ^{1, 2, 3}

KERRY J. VAHALA ^{1, 2, 3}

Hide authors affiliations Show authors affiliations: 3 affiliations

Department of Applied Physics, MC 128-95, California Institute of Technology, Pasadena, CA 91125, USA. |

Division of Biology, MC 139-74, California Institute of Technology, Pasadena, CA 91125, USA. |

Division of Chemistry and Chemical Engineering, MC 210-41, California Institute of Technology, Pasadena, CA 91125, USA. |

Publication type: Journal Article

Publication date: 2007-08-10

American Association for the Advancement of Science (AAAS)

Science

scimago Q1

wos Q1

SJR: 10.416

CiteScore: 48.4

Impact factor: 45.8

ISSN: 00368075, 10959203

DOI: 10.1126/science.1145002

Copy DOI

PubMed ID: 17615303

Multidisciplinary

Abstract

Current single-molecule detection techniques require labeling the target molecule. We report a highly specific and sensitive optical sensor based on an ultrahigh quality (Q) factor (Q > 108) whispering-gallery microcavity. The silica surface is functionalized to bind the target molecule; binding is detected by a resonant wavelength shift. Single-molecule detection is confirmed by observation of single-molecule binding events that shift the resonant frequency, as well as by the statistics for these shifts over many binding events. These shifts result from a thermo-optic mechanism. Additionally, label-free, single-molecule detection of interleukin-2 was demonstrated in serum. These experiments demonstrate a dynamic range of 1012 in concentration, establishing the microcavity as a sensitive and versatile detector.

Found

4 citations

Popov Vladimir

245 publications, 1 429 citations, 1 review

h-index: 17

Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences

1 citation

Makarov Sergey

DSc in Physics and Mathematics, professor

411 publications, 7 704 citations, 62 reviews

h-index: 48

ITMO University

Harbin Engineering University

1 citation

Ponomarenko Sergey

🥼 🤝

DSc in Chemistry, associate member of the Russian Academy of Sciences

261 publications, 6 113 citations, 64 reviews

h-index: 41

Lomonosov Moscow State University

Enikolopov Institute of Synthetic Polymeric Materials of the Russian Academy of Sciences

1 citation

Napolskii Kirill

🤝

PhD in Chemistry

159 publications, 2 629 citations, 13 reviews

h-index: 29

Lomonosov Moscow State University

Research interests

Electrochemistry

Nanowires

1 citation

Chernov Alexander

🥼 🤝

DSc in Physics and Mathematics

147 publications, 2 647 citations, 7 reviews

h-index: 29

Moscow Institute of Physics and Technology

Russian Quantum Center

Research interests

1D Materials

2D Materials

Electronic magnetic resonance

1 citation

Shuklin Fedor

🤝

PhD in Physics and Mathematics

11 publications, 48 citations

h-index: 3

University of Southern Denmark

Research interests

Condensed matter physics

Photonics

Theoretical physics

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GOST |

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GOST Copy

Armani A. M. et al. Label-Free, Single-Molecule Detection with Optical Microcavities // Science. 2007. Vol. 317. No. 5839. pp. 783-787.

GOST all authors (up to 50) Copy

Armani A. M., Kulkarni R. P., Fraser S. E., Flagan R. C., VAHALA K. J. Label-Free, Single-Molecule Detection with Optical Microcavities // Science. 2007. Vol. 317. No. 5839. pp. 783-787.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.1126/science.1145002

UR - https://doi.org/10.1126/science.1145002

TI - Label-Free, Single-Molecule Detection with Optical Microcavities

T2 - Science

AU - Armani, Andrea M

AU - Kulkarni, Rajan P

AU - Fraser, Scott E.

AU - Flagan, Richard C.

AU - VAHALA, KERRY J.

PY - 2007

DA - 2007/08/10

PB - American Association for the Advancement of Science (AAAS)

SP - 783-787

IS - 5839

VL - 317

PMID - 17615303

SN - 0036-8075

SN - 1095-9203

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2007_Armani,

author = {Andrea M Armani and Rajan P Kulkarni and Scott E. Fraser and Richard C. Flagan and KERRY J. VAHALA},

title = {Label-Free, Single-Molecule Detection with Optical Microcavities},

journal = {Science},

year = {2007},

volume = {317},

publisher = {American Association for the Advancement of Science (AAAS)},

month = {aug},

url = {https://doi.org/10.1126/science.1145002},

number = {5839},

pages = {783--787},

doi = {10.1126/science.1145002}

}

MLA

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MLA Copy

Armani, Andrea M., et al. “Label-Free, Single-Molecule Detection with Optical Microcavities.” Science, vol. 317, no. 5839, Aug. 2007, pp. 783-787. https://doi.org/10.1126/science.1145002.

Publisher

American Association for the Advancement of Science (AAAS)

Journal

Science

scimago Q1

wos Q1

SJR

10.416

CiteScore

48.4

Impact factor

45.8

ISSN

00368075 (Print)

10959203 (Electronic)