Open Access
Science, volume 317, issue 5839, pages 783-787
Label-Free, Single-Molecule Detection with Optical Microcavities
Andrea M Armani
1, 2, 3
,
Rajan P Kulkarni
1, 2, 3
,
Scott E. Fraser
1, 2, 3
,
Richard C. Flagan
1, 2, 3
,
KERRY J. VAHALA
1, 2, 3
Publication type: Journal Article
Publication date: 2007-08-10
Multidisciplinary
Abstract
Current single-molecule detection techniques require labeling the target molecule. We report a highly specific and sensitive optical sensor based on an ultrahigh quality (Q) factor (Q > 108) whispering-gallery microcavity. The silica surface is functionalized to bind the target molecule; binding is detected by a resonant wavelength shift. Single-molecule detection is confirmed by observation of single-molecule binding events that shift the resonant frequency, as well as by the statistics for these shifts over many binding events. These shifts result from a thermo-optic mechanism. Additionally, label-free, single-molecule detection of interleukin-2 was demonstrated in serum. These experiments demonstrate a dynamic range of 1012 in concentration, establishing the microcavity as a sensitive and versatile detector.
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Armani A. M. et al. Label-Free, Single-Molecule Detection with Optical Microcavities // Science. 2007. Vol. 317. No. 5839. pp. 783-787.
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Armani A. M., Kulkarni R. P., Fraser S. E., Flagan R. C., VAHALA K. J. Label-Free, Single-Molecule Detection with Optical Microcavities // Science. 2007. Vol. 317. No. 5839. pp. 783-787.
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TY - JOUR
DO - 10.1126/science.1145002
UR - https://doi.org/10.1126/science.1145002
TI - Label-Free, Single-Molecule Detection with Optical Microcavities
T2 - Science
AU - Armani, Andrea M
AU - Kulkarni, Rajan P
AU - Fraser, Scott E.
AU - Flagan, Richard C.
AU - VAHALA, KERRY J.
PY - 2007
DA - 2007/08/10
PB - American Association for the Advancement of Science (AAAS)
SP - 783-787
IS - 5839
VL - 317
SN - 0036-8075
SN - 1095-9203
ER -
Cite this
BibTex
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@article{2007_Armani,
author = {Andrea M Armani and Rajan P Kulkarni and Scott E. Fraser and Richard C. Flagan and KERRY J. VAHALA},
title = {Label-Free, Single-Molecule Detection with Optical Microcavities},
journal = {Science},
year = {2007},
volume = {317},
publisher = {American Association for the Advancement of Science (AAAS)},
month = {aug},
url = {https://doi.org/10.1126/science.1145002},
number = {5839},
pages = {783--787},
doi = {10.1126/science.1145002}
}
Cite this
MLA
Copy
Armani, Andrea M., et al. “Label-Free, Single-Molecule Detection with Optical Microcavities.” Science, vol. 317, no. 5839, Aug. 2007, pp. 783-787. https://doi.org/10.1126/science.1145002.