Open Access
Science, volume 339, issue 6121, pages 819-823
Multiplex Genome Engineering Using CRISPR/Cas Systems
Publication type: Journal Article
Publication date: 2013-01-03
PubMed ID:
23287718
Multidisciplinary
Abstract
Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost). Cong et al. (p. 819, published online 3 January) and Mali et al. (p. 823, published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells. A bacterial genome defense system is adapted to function as a genome-editing tool in mammalian cells. [Also see Perspective by van der Oost] Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences can be encoded into a single CRISPR array to enable simultaneous editing of several sites within the mammalian genome, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
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Cong L. et al. Multiplex Genome Engineering Using CRISPR/Cas Systems // Science. 2013. Vol. 339. No. 6121. pp. 819-823.
GOST all authors (up to 50)
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Cong L., Ran F. A., Cox D., Lin S., Barretto R., Habib N., Hsu P. D., Wu X., Jiang W., Marraffini L. A., Zhang F. Multiplex Genome Engineering Using CRISPR/Cas Systems // Science. 2013. Vol. 339. No. 6121. pp. 819-823.
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TY - JOUR
DO - 10.1126/science.1231143
UR - https://doi.org/10.1126%2Fscience.1231143
TI - Multiplex Genome Engineering Using CRISPR/Cas Systems
T2 - Science
AU - Cong, L.
AU - Ran, F A
AU - Cox, D.
AU - Lin, S.
AU - Barretto, R.
AU - Habib, N.
AU - Hsu, P. D.
AU - Wu, X
AU - Jiang, W.
AU - Marraffini, L A
AU - Zhang, F.
PY - 2013
DA - 2013/01/03 00:00:00
PB - American Association for the Advancement of Science (AAAS)
SP - 819-823
IS - 6121
VL - 339
PMID - 23287718
SN - 0036-8075
SN - 1095-9203
ER -
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@article{2013_Cong,
author = {L. Cong and F A Ran and D. Cox and S. Lin and R. Barretto and N. Habib and P. D. Hsu and X Wu and W. Jiang and L A Marraffini and F. Zhang},
title = {Multiplex Genome Engineering Using CRISPR/Cas Systems},
journal = {Science},
year = {2013},
volume = {339},
publisher = {American Association for the Advancement of Science (AAAS)},
month = {jan},
url = {https://doi.org/10.1126%2Fscience.1231143},
number = {6121},
pages = {819--823},
doi = {10.1126/science.1231143}
}
Cite this
MLA
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Cong, L., et al. “Multiplex Genome Engineering Using CRISPR/Cas Systems.” Science, vol. 339, no. 6121, Jan. 2013, pp. 819-823. https://doi.org/10.1126%2Fscience.1231143.