Open Access
Science, volume 360, issue 6385, pages 219-222
Structure of the nuclear exosome captured on a maturing preribosome
1
Department of Structural Cell Biology, Max Planck Institute (MPI) for Biochemistry, Munich, Germany.
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Publication type: Journal Article
Publication date: 2018-04-13
Multidisciplinary
Abstract
The RNA exosome captured in action The RNA exosome, a major RNA degradation machine, processes ribosomal RNA (rRNA) precursors and is directly coupled to the protein synthesis machine, the ribosome. Using cryo–electron microscopy, Schuller et al. investigated the structure of the precursor large ribosomal subunit from yeast with unprocessed rRNA in complex with the RNA exosome. The structure captures a snapshot of two molecular machines transiently interacting and explains how the RNA exosome acts on an authentic physiological substrate and remodels the large subunit during ribosome maturation. Science, this issue p. 219 The exosome forms a single structural and functional unit with its preribosomal substrate to catalyze ribosomal RNA processing and ribosome maturation. The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo–electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60S) during 7S-to-5.8S rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled “foot” structure of the pre-60S particle, which harbors the 5.8S rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25S rRNA, captures the 3′ extension of the 5.8S rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60S substrate to process rRNA during ribosome maturation.
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GOST
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Schuller J. C. et al. Structure of the nuclear exosome captured on a maturing preribosome // Science. 2018. Vol. 360. No. 6385. pp. 219-222.
GOST all authors (up to 50)
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Schuller J. C., Falk S., Fromm L., Hurt E., Conti E. Structure of the nuclear exosome captured on a maturing preribosome // Science. 2018. Vol. 360. No. 6385. pp. 219-222.
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TY - JOUR
DO - 10.1126/science.aar5428
UR - https://doi.org/10.1126%2Fscience.aar5428
TI - Structure of the nuclear exosome captured on a maturing preribosome
T2 - Science
AU - Schuller, Jan C.
AU - Falk, Sebastian
AU - Fromm, Lisa
AU - Hurt, Ed
AU - Conti, E.
PY - 2018
DA - 2018/04/13 00:00:00
PB - American Association for the Advancement of Science (AAAS)
SP - 219-222
IS - 6385
VL - 360
SN - 0036-8075
SN - 1095-9203
ER -
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@article{2018_Schuller,
author = {Jan C. Schuller and Sebastian Falk and Lisa Fromm and Ed Hurt and E. Conti},
title = {Structure of the nuclear exosome captured on a maturing preribosome},
journal = {Science},
year = {2018},
volume = {360},
publisher = {American Association for the Advancement of Science (AAAS)},
month = {apr},
url = {https://doi.org/10.1126%2Fscience.aar5428},
number = {6385},
pages = {219--222},
doi = {10.1126/science.aar5428}
}
Cite this
MLA
Copy
Schuller, Jan C., et al. “Structure of the nuclear exosome captured on a maturing preribosome.” Science, vol. 360, no. 6385, Apr. 2018, pp. 219-222. https://doi.org/10.1126%2Fscience.aar5428.