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Open access
Molecular and Cellular Biology, volume 34, issue 15, pages 2786-2799

Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells

Zhdanova N.S. 1
Draskovic I 2
Minina J M 1
Karamysheva T.V. 1
Novo C L 2
Liu W. Y. 2
Porreca R M 2
GIBAUD A. 2
Zvereva M E 3
Rubtsov N. B. 1
Londoño Vallejo A 2
2
 
Telomere & Cancer Laboratory, Equipe Labelisée Ligue Nationale contre le Cancer, Institut Curie, Paris, and UPMC University Paris, Paris, France
Publication typeJournal Article
Publication date2014-08-01
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor5.3
ISSN02707306, 10985549
PubMed ID:  24842907
Molecular Biology
Cell Biology
Abstract
ABSTRACT

The telomere structure in the Iberian shrew Sorex granarius is characterized by unique, striking features, with short arms of acrocentric chromosomes carrying extremely long telomeres (up to 300 kb) with interspersed ribosomal DNA (rDNA) repeat blocks. In this work, we investigated the telomere physiology of S. granarius fibroblast cells and found that telomere repeats are transcribed on both strands and that there is no telomere-dependent senescence mechanism. Although telomerase activity is detectable throughout cell culture and appears to act on both short and long telomeres, we also discovered that signatures of a recombinogenic activity are omnipresent, including telomere-sister chromatid exchanges, formation of alternative lengthening of telomeres (ALT)-associated PML-like bodies, production of telomere circles, and a high frequency of telomeres carrying marks of a DNA damage response. Our results suggest that recombination participates in the maintenance of the very long telomeres in normal S. granarius fibroblasts. We discuss the possible interplay between the interspersed telomere and rDNA repeats in the stabilization of the very long telomeres in this organism.

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Zhdanova N. et al. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells // Molecular and Cellular Biology. 2014. Vol. 34. No. 15. pp. 2786-2799.
GOST all authors (up to 50) Copy
Zhdanova N., Draskovic I., Minina J. M., Karamysheva T., Novo C. L., Liu W. Y., Porreca R. M., GIBAUD A., Zvereva M. E., Skvortsov D. A., Rubtsov N. B., Londoño Vallejo A. Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells // Molecular and Cellular Biology. 2014. Vol. 34. No. 15. pp. 2786-2799.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1128/MCB.01697-13
UR - https://doi.org/10.1128%2FMCB.01697-13
TI - Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells
T2 - Molecular and Cellular Biology
AU - Zhdanova, N.S.
AU - Draskovic, I
AU - Minina, J M
AU - Karamysheva, T.V.
AU - Novo, C L
AU - Liu, W. Y.
AU - Porreca, R M
AU - GIBAUD, A.
AU - Zvereva, M E
AU - Skvortsov, D A
AU - Rubtsov, N. B.
AU - Londoño Vallejo, A
PY - 2014
DA - 2014/08/01 00:00:00
PB - American Society for Microbiology
SP - 2786-2799
IS - 15
VL - 34
PMID - 24842907
SN - 0270-7306
SN - 1098-5549
ER -
BibTex |
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BibTex Copy
@article{2014_Zhdanova,
author = {N.S. Zhdanova and I Draskovic and J M Minina and T.V. Karamysheva and C L Novo and W. Y. Liu and R M Porreca and A. GIBAUD and M E Zvereva and D A Skvortsov and N. B. Rubtsov and A Londoño Vallejo},
title = {Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells},
journal = {Molecular and Cellular Biology},
year = {2014},
volume = {34},
publisher = {American Society for Microbiology},
month = {aug},
url = {https://doi.org/10.1128%2FMCB.01697-13},
number = {15},
pages = {2786--2799},
doi = {10.1128/MCB.01697-13}
}
MLA
Cite this
MLA Copy
Zhdanova, N.S., et al. “Recombinogenic telomeres in diploid Sorex granarius (Soricidae, Eulipotyphla) fibroblast cells.” Molecular and Cellular Biology, vol. 34, no. 15, Aug. 2014, pp. 2786-2799. https://doi.org/10.1128%2FMCB.01697-13.
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