Open Access
mSystems, volume 3, issue 2
The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
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Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Sao Paulo, Brazil
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Publication type: Journal Article
Publication date: 2018-04-24
PubMed ID:
29629418
Biochemistry
Computer Science Applications
Molecular Biology
Genetics
Microbiology
Physiology
Ecology, Evolution, Behavior and Systematics
Modeling and Simulation
Abstract
Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) gene clusters under laboratory cultivation. ABSTRACT Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) gene clusters under laboratory cultivation. Further progress in compound detection, such as the development of the tandem mass spectrometry (MS/MS) molecular networking approach, has contributed to the discovery of novel bacterial natural products. The latter can be applied directly to bacterial crude extracts for identifying and dereplicating known compounds, therefore assisting the prioritization of extracts containing novel natural products, for example. In our opinion, these three approaches—genome mining, silent pathway induction, and MS-based molecular networking—compose the tripod for modern bacterial natural product discovery and will be discussed in this perspective.
Citations by journals
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Marine Drugs
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Marine Drugs
4 publications, 14.81%
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Molecules
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Molecules
2 publications, 7.41%
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Journal of Pharmaceutical Analysis
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Journal of Pharmaceutical Analysis, 1, 3.7%
Journal of Pharmaceutical Analysis
1 publication, 3.7%
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Metabolites
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Metabolites
1 publication, 3.7%
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Frontiers in Marine Science
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Frontiers in Marine Science
1 publication, 3.7%
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Frontiers in Microbiology
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Frontiers in Microbiology
1 publication, 3.7%
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World Journal of Microbiology and Biotechnology
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World Journal of Microbiology and Biotechnology
1 publication, 3.7%
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Biotechnology Letters
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Biotechnology Letters
1 publication, 3.7%
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Journal of Biotechnology
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Journal of Biotechnology
1 publication, 3.7%
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Cell
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Cell
1 publication, 3.7%
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Journal of Microbiological Methods
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Journal of Microbiological Methods
1 publication, 3.7%
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Current Opinion in Chemical Biology
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Current Opinion in Chemical Biology
1 publication, 3.7%
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Journal of Invertebrate Pathology
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Journal of Invertebrate Pathology
1 publication, 3.7%
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Acta Pharmaceutica Sinica B
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Acta Pharmaceutica Sinica B
1 publication, 3.7%
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ChemBioChem
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ChemBioChem
1 publication, 3.7%
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Israel Journal of Chemistry
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Israel Journal of Chemistry
1 publication, 3.7%
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Natural Product Reports
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Natural Product Reports
1 publication, 3.7%
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Bioinformatics
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Bioinformatics
1 publication, 3.7%
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Applied and Environmental Soil Science
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Applied and Environmental Soil Science
1 publication, 3.7%
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 25.93%
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Elsevier
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Elsevier
6 publications, 22.22%
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Frontiers Media S.A.
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Frontiers Media S.A.
2 publications, 7.41%
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Springer Nature
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Springer Nature
2 publications, 7.41%
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Wiley
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Wiley
2 publications, 7.41%
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Xi'an Jiaotong University
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Xi'an Jiaotong University, 1, 3.7%
Xi'an Jiaotong University
1 publication, 3.7%
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Royal Society of Chemistry (RSC)
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Royal Society of Chemistry (RSC)
1 publication, 3.7%
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Oxford University Press
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Oxford University Press
1 publication, 3.7%
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Hindawi Limited
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Hindawi Limited
1 publication, 3.7%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Trivella D. B. B., De Felício R. The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking // mSystems. 2018. Vol. 3. No. 2.
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Trivella D. B. B., De Felício R. The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking // mSystems. 2018. Vol. 3. No. 2.
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TY - JOUR
DO - 10.1128/mSystems.00160-17
UR - https://doi.org/10.1128%2FmSystems.00160-17
TI - The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
T2 - mSystems
AU - Trivella, D. B. B.
AU - De Felício, Rafael
PY - 2018
DA - 2018/04/24 00:00:00
PB - American Society for Microbiology
IS - 2
VL - 3
PMID - 29629418
SN - 2379-5077
ER -
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@article{2018_Trivella,
author = {D. B. B. Trivella and Rafael De Felício},
title = {The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking},
journal = {mSystems},
year = {2018},
volume = {3},
publisher = {American Society for Microbiology},
month = {apr},
url = {https://doi.org/10.1128%2FmSystems.00160-17},
number = {2},
doi = {10.1128/mSystems.00160-17}
}