Russian Journal of Genetics, volume 60, issue 11, pages 1588-1591

The Complete Chloroplast Genome of Lessonia nigrescens and Phylogenetic Analysis

P.-N. Wang 1
X. Y. Wang 1
H.C Zhao 1
Y Y He 1
C. F. Qu 1, 2
J L Miao 1, 2, 3
1
 
First Institute of Oceanography, Ministry of Natural Resources, Qingdao, China
2
 
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Publication typeJournal Article
Publication date2024-11-25
scimago Q4
wos Q4
SJR0.185
CiteScore1.0
Impact factor0.6
ISSN10227954, 16083369
Abstract
Lessonia nigrescens belongs to the genus Lessonia of the family Lessoniaceae, a macroalgae that grows in the rocky intertidal zones and shallow subtidal waters of temperate Pacific South American coasts. In this study, we determined the complete chloroplast genome of L. nigrescens the first time and analyzed its evolutionary relationship. The chloroplast genome of L. nigrescens was sequenced in full using Illumina HiSeq 2500. The total length of chloroplast genome was 130,317 bp, containing a large single-copy region (LSC, 76,610 bp), a small single-copy region (SSC, 42,911 bp) and a pair of inverted repeat regions (IRs, 10,796 bp). The circular genome consisted of 140 protein-coding genes, 28 tRNA genes and 6 rRNA genes, with a total of 174. Phylogenetic analysis confirmed the position of L. nigrescens within the Phaeophyta.
Liu T., Zuo Z., He Y., Miao J., Yu J., Qu C.
2023-10-30 citations by CoLab: 2
Hao J., Lu Y., Dang M., Xia R., Xu L., Zhu Z., Yu Y.
2023-10-03 citations by CoLab: 3
Nardelli A.E., Visch W., Wright J.T., Hurd C.L.
Journal of Applied Phycology scimago Q2 wos Q1
2023-04-15 citations by CoLab: 8 Abstract  
Abstract Lessonia (order Laminariales) is a kelp genus restricted to the temperate southern hemisphere, where species form dense forests from the low intertidal to 25 m depth at wave exposed sites. Lessonia spp. are among the most harvested kelps globally due to their importance in providing raw materials for food, cosmetics, bioactive and biomedical industries. Over-harvesting of natural beds can negatively affect Lessonia populations and the many species that depend on these habitats, including commercially important fish and molluscs, but good harvest management plans reduce these impacts on natural Lessonia stocks. However, the increasing demand for raw materials will likely only be met by aquaculture for which Lessonia shows high potential in pilot scale studies undertaken in Chile, New Zealand, and Australia. In this concise review, we highlight the current knowledge of Lessonia spp. taxonomy and distribution, life history, ecology and ecosystem services, wild harvest, aquaculture, and commercial applications. We discuss future research directions.
Zou P., Lu X., Zhao H., Yuan Y., Meng L., Zhang C., Li Y.
Frontiers in Plant Science scimago Q1 wos Q1 Open Access
2019-01-31 citations by CoLab: 90 PDF Abstract  
Soil salinity reduces plant growth and is a major factor that causes decreased agricultural productivity worldwide. Seaweed polysaccharides promote crop growth and improve plant resistance to abiotic stress. In this study, polysaccharides from brown seaweed Lessonia nigrescens (LNP) were extracted and further separated and fractionated. Two acidic polysaccharides (LNP-1 and LNP-2) from crude LNP were obtained and characterized. The latter had a lower molecular weight (40.2 kDa) than the former (63.9 kDa), but had higher uronic acid and sulfate content. Crude LNP and LNP-2 were composed of mannose, glucuronic acid, fucose and xylose, whereas LNP-1 has little mannose. Moreover, the effects on plant salt tolerance of the three polysaccharides were investigated. The results showed that crude LNP, LNP-1 and LNP-2 promoted the growth of plants, decreased membrane lipid peroxidation, increased the chlorophyll content, improved antioxidant activities, and coordinated the efflux and compartmentation of intracellular ion. All three polysaccharides could induce plant resistance to salt stress, but LNP-2 was more effective than the other two. It can be concluded that both molecular weight and sulfate degree contribute to salt resistance capability of polysaccharides derived from L. nigrescens.
Zhao C., Yang C., Chen M., Lv X., Liu B., Yi L., Cornara L., Wei M., Yang Y., Tundis R., Xiao J.
2018-01-12 citations by CoLab: 52 Abstract  
In this study, the antidiabetic activity of Lessonia nigrescens ethanolic extract (LNE) is investigated in streptozotocin (SZT)-induced type 2 diabetic mice fed with a high-sucrose/high-fat diet.Ultra high performance liquid chromatography coupled with photo-DAD and electospray ionization-mass spectrometry (ESI-MS) is employed to analyze the major compounds in LNE. The components of the intestinal microflora in type 2 diabetic mice are analyzed by high-throughput next-generation 16S rRNA gene sequencing. Fasting blood glucose levels in diabetic mice are significantly decreased after LNE administration. The histology reveals that LNE could protect the cellular architecture of liver and kidney. LNE treatment significantly increases Bacteroidetes and decreases Firmicutes populations in intestinal microflora. Specifically, It could selectively enrich the amounts of beneficial bacteria, Barnesiella, as well as reduce the abundances of Clostridium and Alistipes. The increased gene and protein expression levels of phosphatidylinositol 3-kinase (PI3K) in the liver are observed in LNE treatment groups, while the expressions of c-Jun N-terminal kinase (JNK) are significantly downregulated.The above findings suggest that LNE could be considered as a functional food for reducing blood glucose and regulating intestinal microflora.
Gutiérrez C., Hansen H.K., Hernández P., Pinilla C.
Chemosphere scimago Q1 wos Q1
2015-11-01 citations by CoLab: 37 Abstract  
Sorption experiments for cadmium removal using two brown macroalgae Lessonia nigrescens and Durvillaea antarctica were carried out. Although both types of algae were capable of retaining cadmium, differences in their performance were observed. The optimum pH was 3.7±0.2, and to achieve the equilibrium, 5 days of contact time were necessary for both biosorbents. The maximum experimental uptake obtained was similar for the two biosorbents: 95.3 mg Cd g(-1) by D. antarctica and 109.5 mg Cd g(-1) by L. nigrescens. The Langmuir model described the equilibrium sorption isotherms very well for both biosorbents and the Lagergren pseudo primer order model described the sorption kinetics for L. nigrescens satisfactorily and the Ho and Mckay pseudo second order model for D. antarctica. It was found that cadmium uptake by D. antarctica was faster than by L. nigrescens.
González A., Beltrán J., Hiriart-Bertrand L., Flores V., de Reviers B., Correa J.A., Santelices B.
Journal of Phycology scimago Q1 wos Q2
2012-07-10 citations by CoLab: 62 Abstract  
The kelp Lessonia nigrescens Bory is the most ecologically and economically important seaweed in rocky intertidal and shallow subtidal habitats along the temperate Pacific South American coasts. Recent molecular studies suggest the existence of two lineages, one (northern lineage) from 17° S to 30° S and a second (central lineage) from 29° S to 41° S. To identify and name these lineages we performed morphological, nomenclatural and field studies. Four external and three internal anatomical traits permitted a morphological separation of the two lineages. The internal structure of both lineages was different from the isolectotype of Lessonia nigrescens. It is therefore concluded that the name Lessonia nigrescens should not be used for the Chilean material. Chordaria spicata Suhr appears as the oldest available name for the central lineage, while Lessonia berteroana Montagne is the oldest name for the northern lineage. In both cases, the type material consisted of small-sized, apical branches of larger plants. The new combination Lessonia spicata (Suhr) Santelices is proposed for the central lineage and we reinstate Lessonia berteroana for the northern lineage. Laminaria scissa Suhr is reduced to synonym of L. spicata. Representative specimens of Lessonia nigrescens were not found during new visits to its type locality in Cape Horn and along Chile. Future studies should verify the status of this species.
Reategui M., Maldonado H., Ly M., Guibal E.
2010-02-14 citations by CoLab: 14 Abstract  
Lessonia nigrescens and Lessonia trabeculata kelps have been tested for the sorption of mercury from aqueous solutions. A pretreatment (using CaCl2) allowed stabilizing the biomass that was very efficient for removing Hg(II) at pH 6–7. Sorption isotherms were described by the Langmuir equation with sorption capacities close to 240–270 mg Hg g−1 at pH 6. The temperature had a negligible effect on the distribution of the metal at equilibrium. The presence of chloride anions had a more marked limiting impact than sulfate and nitrate anions. The uptake kinetics were modeled using the pseudo-second-order equation that fitted better experimental data than the pseudo-first-order equation. The particle size hardly influenced sorption isotherms and uptake kinetics, indicating that sorption occurs in the whole mass of the biosorbent and that intraparticle mass transfer resistance was not the limiting rate. Varying the sorbent dosage and the initial metal concentration influenced the equilibrium, but the kinetic parameters were not drastically modified. Metal can be eluted with hydrochloric acid, citric acid, or acidic KI solutions.
FAUGERON S., VELIZ D., PERALTA G., TAPIA J., TELLIER F., BILLOT C., MARTINEZ E.
Molecular Ecology Resources scimago Q1 wos Q1
2009-05-01 citations by CoLab: 7 Abstract  
A total of nine microsatellite loci were isolated and characterized in the Chilean kelp Lessonia nigrescens Bory. Using two different enriched libraries, we observed 1-14 alleles per locus in two samples of 21 kelp individuals each. The observed heterozygosities ranged from 0.05 to 0.80 and all loci are in Hardy-Weinberg equilibrium for one or both samples. Seventeen samples collected from different sites showed high allele diversity along the species distribution. The variation detected at these markers is currently being used for the study of populations of Lessonia nigrescens at different geographical scales.
ANDRADE S., CONTRERAS L., MOFFETT J., CORREA J.
Aquatic Toxicology scimago Q1 wos Q1
2006-07-20 citations by CoLab: 43 Abstract  
Juvenile individuals of the brown kelp Lessonia nigrescens were exposed to a coastal environment chronically impacted by copper mine wastes and currently displaying more than 250 nM of total dissolved copper. The kinetic of copper accumulation in the intra and extracellular compartments was determined and correlated to the oxidative burst resulting from copper-mediated oxidative stress. Accumulation involved an initial adsorption onto the outer cell wall followed by a slower uptake into the cells. A linear pattern of copper uptake over time was found during the first 52 h of exposure, and a steady state was reached at 76 h. The resulting oxidative stress was found to be inefficiently attenuated, and the intracellular level of copper remained sufficiently high to determine a persistently higher than normal level of reactive oxygen species (ROS). Thus, our results strongly suggest that, in L. nigrescens, copper needs to reach an intracellular threshold before oxidative burst develops. Furthermore, it was found that the high ROS levels generated by copper accumulation within the cells persists after the oxidative burst has ceased, suggesting a limited capacity of the algal tissue to buffer the increases of ROS caused by the environmental copper levels.
Wächter G.A., Franzblau S.G., Montenegro G., Hoffmann J.J., Maiese W.M., Timmermann B.N.
Journal of Natural Products scimago Q1 wos Q1
2001-10-26 citations by CoLab: 39 Abstract  
Assay-guided fractionation of an antitubercular extract obtained from Lessonia nigrescens yielded the phytosterol saringosterol as its active component. No appreciable toxicity against Vero cells was observed for this compound. Saringosterol was also synthesized by oxidation of fucosterol. The MIC values for antitubercular activity of saringosterol and its 24S and 24R epimers were determined as 0.25, 1, and 0.125 microg/mL.

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