Pleiades Publishing
Russian Journal of Bioorganic Chemistry
,  volume 50 ,  issue 2 ,  pages 459-466

E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells

N Deeb 1
, 
G. D. Otinov 1
, 
A. Mohamed 1
, 
E. R. Gandalipov 1
, 
E. A. Kovtunov 1, 2
, 
E I Koshel 1
1
 
2
 
Penn Sate College of Medicine, Hershey Medical Center, Hershey, USA | 
Publication type: Journal Article
Publication date: 2024-04-09
Pleiades Publishing
scimago Q4
wos Q3
SJR: 0.267
CiteScore: 1.9
Impact factor: 1.7
ISSN: 10681620, 1608330X, 15739163
Organic Chemistry
Biochemistry
Abstract
Objective: In recent years, bactofection—bacteria-mediated DNA transfer into mammalian cells is considered a promising approach to cancer gene therapy. Results and Discussion: In this study, we first attempted to develop a probiotic strain Escherichia coli Nissle 1917 for transferring shuttle plasmid into colorectal carcinoma cells HCT116. For this purpose, the strain was modified by inv and hly genes from Yersinia enterocolitica and Listeria monocytogenes. Conclusions: Although the efficiency of HCT116 transfection by GFP-plasmid was exceptionally low, the results demonstrated the potential use the modified probiotic strain for bactofection.
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Deeb N. et al. E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells // Russian Journal of Bioorganic Chemistry. 2024. Vol. 50. No. 2. pp. 459-466.
GOST all authors (up to 50) Copy
Deeb N., Otinov G. D., Mohamed A., Gandalipov E. R., Kovtunov E. A., Koshel E. I. E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells // Russian Journal of Bioorganic Chemistry. 2024. Vol. 50. No. 2. pp. 459-466.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/s1068162024110451
UR - https://link.springer.com/10.1134/S1068162024110451
TI - E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells
T2 - Russian Journal of Bioorganic Chemistry
AU - Deeb, N
AU - Otinov, G. D.
AU - Mohamed, A.
AU - Gandalipov, E. R.
AU - Kovtunov, E. A.
AU - Koshel, E I
PY - 2024
DA - 2024/04/09
PB - Pleiades Publishing
SP - 459-466
IS - 2
VL - 50
SN - 1068-1620
SN - 1608-330X
SN - 1573-9163
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Deeb,
author = {N Deeb and G. D. Otinov and A. Mohamed and E. R. Gandalipov and E. A. Kovtunov and E I Koshel},
title = {E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2024},
volume = {50},
publisher = {Pleiades Publishing},
month = {apr},
url = {https://link.springer.com/10.1134/S1068162024110451},
number = {2},
pages = {459--466},
doi = {10.1134/s1068162024110451}
}
MLA
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MLA Copy
Deeb, N., et al. “E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells.” Russian Journal of Bioorganic Chemistry, vol. 50, no. 2, Apr. 2024, pp. 459-466. https://link.springer.com/10.1134/S1068162024110451.