Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study
O. A. Tolicheva
1
,
M. S. Bidzhieva
1
,
P. S. Kasatskiy
1
,
V I Marina
2, 3
,
P V Sergiev
2, 3
,
A L Konevega
1, 4, 5
,
A Paleskava
1, 4
1
Publication type: Journal Article
Publication date: 2024-06-01
scimago Q4
wos Q4
SJR: 0.150
CiteScore: 0.7
Impact factor: 0.5
ISSN: 26351676, 26351684
Abstract
The development of simple, readily available, and sensitive methods for studying bacterial protein synthesis is an important fundamental and applied task. We recently demonstrated the possibility of visualizing short BODIPY-labeled phenylalanine- and leucine-containing peptides using urea-polyacrylamide gel electrophoresis under denaturing conditions. In this work, we expand the range of test sequences and included peptides with valine, lysine, and the modified amino acid εNH2-DOTA-lysine. The described method can also be used to study the mechanism of translation inhibition, as shown by elaboration of the specific action of the antibiotics etamycin A and viomycin.
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Total citations:
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Citations from 2024:
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Tolicheva O. A. et al. Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study // Nanobiotechnology Reports. 2024. Vol. 19. No. 3. pp. 423-431.
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Tolicheva O. A., Bidzhieva M. S., Kasatskiy P. S., Marina V. I., Sergiev P. V., Konevega A. L., Paleskava A. Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study // Nanobiotechnology Reports. 2024. Vol. 19. No. 3. pp. 423-431.
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RIS
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TY - JOUR
DO - 10.1134/s263516762460127x
UR - https://link.springer.com/10.1134/S263516762460127X
TI - Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study
T2 - Nanobiotechnology Reports
AU - Tolicheva, O. A.
AU - Bidzhieva, M. S.
AU - Kasatskiy, P. S.
AU - Marina, V I
AU - Sergiev, P V
AU - Konevega, A L
AU - Paleskava, A
PY - 2024
DA - 2024/06/01
PB - Pleiades Publishing
SP - 423-431
IS - 3
VL - 19
SN - 2635-1676
SN - 2635-1684
ER -
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BibTex (up to 50 authors)
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@article{2024_Tolicheva,
author = {O. A. Tolicheva and M. S. Bidzhieva and P. S. Kasatskiy and V I Marina and P V Sergiev and A L Konevega and A Paleskava},
title = {Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study},
journal = {Nanobiotechnology Reports},
year = {2024},
volume = {19},
publisher = {Pleiades Publishing},
month = {jun},
url = {https://link.springer.com/10.1134/S263516762460127X},
number = {3},
pages = {423--431},
doi = {10.1134/s263516762460127x}
}
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MLA
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Tolicheva, O. A., et al. “Separation of Short Fluorescently Labeled Peptides by Gel Electrophoresis for an In Vitro Translation Study.” Nanobiotechnology Reports, vol. 19, no. 3, Jun. 2024, pp. 423-431. https://link.springer.com/10.1134/S263516762460127X.