Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase
Publication type: Journal Article
Publication date: 2016-03-30
scimago Q3
wos Q4
SJR: 0.294
CiteScore: 1.7
Impact factor: 1.1
ISSN: 00036838, 16083024
PubMed ID:
27266256
Biochemistry
Applied Microbiology and Biotechnology
Abstract
Fluorescent and optical spectroscopy were used to study the interaction of alcohol dehydrogenase (ADH) with negatively charged polystyrene sulfonate (PSS) and dextran sulfate (DS), as well as positively charged poly(diallyldimethylammonium) (PDADMA). As found, DS and PDADMA did not affect the structural and catalytic enzyme properties. In contrast, PSS slightly decreased the protein self-fluorescence over 1 h of incubation, which is associated with partial destruction of its quaternary (globular) structure. Investigation of the ADH activity with and without PSS showed its dependency on the incubation time and the PSS presence. Sodium chloride (2.0 and 0.2 M) or ammonium sulfate (0.1 M) added to the reaction mixture did not completely protect the enzyme quaternary structure from the PSS action. However ammonium sulfate or 0.2 M sodium chloride stabilized the enzyme and partially inhibited the negative PSS effect.
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Dubrovsky A. V. et al. Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase // Applied Biochemistry and Microbiology. 2016. Vol. 52. No. 2. pp. 233-236.
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Dubrovsky A. V., Musin E. V., Kim A. L., Tikhonenko S. A. Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase // Applied Biochemistry and Microbiology. 2016. Vol. 52. No. 2. pp. 233-236.
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TY - JOUR
DO - 10.1134/S0003683816020046
UR - http://link.springer.com/10.1134/S0003683816020046
TI - Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase
T2 - Applied Biochemistry and Microbiology
AU - Dubrovsky, A. V.
AU - Musin, E. V.
AU - Kim, A. L.
AU - Tikhonenko, S. A.
PY - 2016
DA - 2016/03/30
PB - Pleiades Publishing
SP - 233-236
IS - 2
VL - 52
PMID - 27266256
SN - 0003-6838
SN - 1608-3024
ER -
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BibTex (up to 50 authors)
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@article{2016_Dubrovsky,
author = {A. V. Dubrovsky and E. V. Musin and A. L. Kim and S. A. Tikhonenko},
title = {Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase},
journal = {Applied Biochemistry and Microbiology},
year = {2016},
volume = {52},
publisher = {Pleiades Publishing},
month = {mar},
url = {http://link.springer.com/10.1134/S0003683816020046},
number = {2},
pages = {233--236},
doi = {10.1134/S0003683816020046}
}
Cite this
MLA
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Dubrovsky, A. V., et al. “Effect of Polyelectrolytes on Catalytic Activity of Alcohol Dehydrogenase.” Applied Biochemistry and Microbiology, vol. 52, no. 2, Mar. 2016, pp. 233-236. http://link.springer.com/10.1134/S0003683816020046.