Applied Biochemistry and Microbiology

, том 56 , издание 5 , страницы 538-543

Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation

S Yu But ¹

S N Dedysh ²

V. O. Popov ³

N. V. Pimenov ²

V. N. Khmelenina ¹

Скрыть аффилиации авторов Показать аффилиации авторов: 3 аффилиации

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research Federal Research Center, Russian Academy of Sciences, Pushchino, Russia

Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН

ФИЦ "Пущинский научный центр биологических исследований" РАН

Winogradsky Institute of Microbiology, Biotechnology Research Center, Russian Academy of Sciences, Moscow, Russia |

Bach Institute of Biochemistry, Biotechnology Research Center, Russian Academy of Sciences, Moscow, Russia |

Тип публикации: Journal Article

Дата публикации: 2020-09-22

Pleiades Publishing

Applied Biochemistry and Microbiology

scimago Q3

wos Q4

БС2

SJR: 0.294

CiteScore: 1.7

Impact factor: 1.1

ISSN: 00036838, 16083024

DOI: 10.1134/S0003683820050063

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Biochemistry

Applied Microbiology and Biotechnology

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Mutant strains of the halotolerant type-I methanotroph Methylotuvimicrobium alcaliphilum 20Z with an inactivated sps gene, which encodes sucrose phosphate synthase, and the deletion of a gene cluster for the synthesis and degradation of glycogen were obtained. Blockage of the synthesis of sucrose and glycogen increased the protein content in cells but slightly reduced the growth rate of the methanotroph grown on methane. It is shown that relatively stable methanotroph growth is possible without synthesis of the storage carbon compounds.

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Бут Сергей

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Институт микробиологии им. С.Н. Виноградского РАН

Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина РАН

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But S. Yu. et al. Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation // Applied Biochemistry and Microbiology. 2020. Vol. 56. No. 5. pp. 538-543.

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But S. Yu., Dedysh S. N., Popov V. O., Pimenov N. V., Khmelenina V. N. Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation // Applied Biochemistry and Microbiology. 2020. Vol. 56. No. 5. pp. 538-543.

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TY - JOUR

DO - 10.1134/S0003683820050063

UR - https://doi.org/10.1134/S0003683820050063

TI - Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation

T2 - Applied Biochemistry and Microbiology

AU - But, S Yu

AU - Dedysh, S N

AU - Popov, V. O.

AU - Pimenov, N. V.

AU - Khmelenina, V. N.

PY - 2020

DA - 2020/09/22

PB - Pleiades Publishing

SP - 538-543

IS - 5

VL - 56

SN - 0003-6838

SN - 1608-3024

ER -

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@article{2020_But,

author = {S Yu But and S N Dedysh and V. O. Popov and N. V. Pimenov and V. N. Khmelenina},

title = {Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation},

journal = {Applied Biochemistry and Microbiology},

year = {2020},

volume = {56},

publisher = {Pleiades Publishing},

month = {sep},

url = {https://doi.org/10.1134/S0003683820050063},

number = {5},

pages = {538--543},

doi = {10.1134/S0003683820050063}

}

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But, S. Yu., et al. “Construction of a Type-I Metanotroph with Reduced Capacity for Glycogen and Sucrose Accumulation.” Applied Biochemistry and Microbiology, vol. 56, no. 5, Sep. 2020, pp. 538-543. https://doi.org/10.1134/S0003683820050063.