Pleiades Publishing
Biochemistry (Moscow)
,  volume 84 ,  issue 4 ,  pages 390-397

Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium

Publication type: Journal Article
Publication date: 2019-04-24
Pleiades Publishing
scimago Q2
wos Q4
SJR: 0.659
CiteScore: 3.8
Impact factor: 2.2
ISSN: 00062979, 16083040
Biochemistry
General Medicine
Abstract
Recombinant malic enzyme from the aerobic methanotroph Methylosinus trichosporium was obtained by heterologous expression in Escherichia coli and purified by affinity metal-chelating chromatography. The homohexameric enzyme of 6×80 kDa catalyzed the reversible reaction of oxidative decarboxylation of malate to pyruvate in the presence of mono- and divalent cations and NADP+ as a cofactor. The kcat/Km ratio indicated much higher catalytic efficiency of the malate decarboxylation reaction as compared with the pyruvate carboxylation reaction. Analysis of the protein sequence revealed that the C-region of the enzyme contains a large domain homologous to phosphoacetyltransferase, but no phosphoacetyl-transferase activity was detected either for a full chimeric malic enzyme or for the C-end fragment obtained as a separate protein. This C-end domain promoted activity of the malic enzyme.
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Rozova O. N. et al. Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium // Biochemistry (Moscow). 2019. Vol. 84. No. 4. pp. 390-397.
GOST all authors (up to 50) Copy
Rozova O. N., Khmelenina V. N., Mustakhimov I. I., But S. Y., Trotsenko Y. A. Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium // Biochemistry (Moscow). 2019. Vol. 84. No. 4. pp. 390-397.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/S0006297919040060
UR - https://doi.org/10.1134/S0006297919040060
TI - Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium
T2 - Biochemistry (Moscow)
AU - Rozova, O. N.
AU - Khmelenina, V. N.
AU - Mustakhimov, I. I.
AU - But, S. Y.
AU - Trotsenko, Yu. A.
PY - 2019
DA - 2019/04/24
PB - Pleiades Publishing
SP - 390-397
IS - 4
VL - 84
PMID - 31228930
SN - 0006-2979
SN - 1608-3040
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2019_Rozova,
author = {O. N. Rozova and V. N. Khmelenina and I. I. Mustakhimov and S. Y. But and Yu. A. Trotsenko},
title = {Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium},
journal = {Biochemistry (Moscow)},
year = {2019},
volume = {84},
publisher = {Pleiades Publishing},
month = {apr},
url = {https://doi.org/10.1134/S0006297919040060},
number = {4},
pages = {390--397},
doi = {10.1134/S0006297919040060}
}
MLA
Cite this
MLA Copy
Rozova, O. N., et al. “Properties of Malic Enzyme from the Aerobic Methanotroph Methylosinus trichosporium.” Biochemistry (Moscow), vol. 84, no. 4, Apr. 2019, pp. 390-397. https://doi.org/10.1134/S0006297919040060.
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