Measurements of IgG Antibodies Adsorption onto Electrospun Nylon-6 Membranes

Biosensors based on electrospun membranes have some advantages over the traditional biosensor devices that include a receptor layer immobilized on a flat support. Due to the large surface area of the electrospun membrane, the number of analyte-binding sites increases but it does not interfere with traditional detection methods (e.g., electrochemical detection). In this study, the adsorption of IgG antibodies onto electrospun nylon-6 membranes was explored. It was shown that at an antibody concentration of 2.5–10 μg/mL, the membrane had a binding capacity of ~100 μg/g and the volume fraction of the adsorbed antibodies was 40–45%. Using laser scanning confocal microscopy, it was found that the adsorbed antibodies were washed from the outer layers of the membrane more efficiently than from the inner layers. The procedure for measuring the binding capacity of membranes introduced in this paper can be a useful tool in developing immunosensors.