Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus
V V Dubrovskaya
1
,
A B Ulitin
2
,
A G Laman
2
,
I.P. Gileva
1
,
N.I. Bormotov
1
,
A. A. Ilyichev
1
,
F A Brovko
2
,
S N Shchelkunov
1
,
E.F. Belanov
1
,
N V Tikunova
1
Publication type: Journal Article
Publication date: 2007-02-26
scimago Q3
wos Q4
SJR: 0.331
CiteScore: 1.6
Impact factor: 1.2
ISSN: 00268933, 16083245, 21689547
Structural Biology
Biophysics
Abstract
A combinatorial immune library of human single-chain antibodies (scAbs) was constructed using the genes coding for the variable domains of the heavy and light chains of human immunoglobulins. The genes were cloned from lymphocytes of four subjects vaccinated with the vaccinia virus (VACV). The library included 3 · 107 independent clones. After enrichment with clones producing scAbs against a recombinant analog of the variola virus envelope protein prA30L, the library was used to select a panel of scAbs binding both prA30L and VACV. All scAbs selected were tested for virus-neutralizing activity, and two scAbs proved to suppress VACV plaque formation in monolayers of Vero E6 cells. The specificity of antigen binding was verified by ELISA and Western blotting. The amino acid sequences of the virus-neutralizing scAbs were determined by sequencing their genes.
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Dubrovskaya V. V. et al. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.
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Dubrovskaya V. V., Ulitin A. B., Laman A. G., Gileva I., Bormotov N., Ilyichev A. A., Brovko F. A., Shchelkunov S. N., Belanov E., Tikunova N. V. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.
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TY - JOUR
DO - 10.1134/S0026893307010207
UR - https://doi.org/10.1134/S0026893307010207
TI - Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus
T2 - Molecular Biology
AU - Dubrovskaya, V V
AU - Ulitin, A B
AU - Laman, A G
AU - Gileva, I.P.
AU - Bormotov, N.I.
AU - Ilyichev, A. A.
AU - Brovko, F A
AU - Shchelkunov, S N
AU - Belanov, E.F.
AU - Tikunova, N V
PY - 2007
DA - 2007/02/26
PB - Pleiades Publishing
SP - 157-167
IS - 1
VL - 41
SN - 0026-8933
SN - 1608-3245
SN - 2168-9547
ER -
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BibTex (up to 50 authors)
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@article{2007_Dubrovskaya,
author = {V V Dubrovskaya and A B Ulitin and A G Laman and I.P. Gileva and N.I. Bormotov and A. A. Ilyichev and F A Brovko and S N Shchelkunov and E.F. Belanov and N V Tikunova},
title = {Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus},
journal = {Molecular Biology},
year = {2007},
volume = {41},
publisher = {Pleiades Publishing},
month = {feb},
url = {https://doi.org/10.1134/S0026893307010207},
number = {1},
pages = {157--167},
doi = {10.1134/S0026893307010207}
}
Cite this
MLA
Copy
Dubrovskaya, V. V., et al. “Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus.” Molecular Biology, vol. 41, no. 1, Feb. 2007, pp. 157-167. https://doi.org/10.1134/S0026893307010207.
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