Molecular Biology, volume 41, issue 1, pages 157-167
Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus
Dubrovskaya V V
1
,
Ulitin A B
2
,
Laman A G
2
,
Gileva I.P.
1
,
Bormotov N.I.
1
,
Ilyichev A. A.
1
,
Brovko F A
2
,
Shchelkunov S N
1
,
Belanov E.F.
1
,
Tikunova N V
1
Publication type: Journal Article
Publication date: 2007-02-26
Journal:
Molecular Biology
Quartile SCImago
Q4
Quartile WOS
Q4
Impact factor: 1.2
ISSN: 00268933, 16083245
Structural Biology
Biophysics
Abstract
A combinatorial immune library of human single-chain antibodies (scAbs) was constructed using the genes coding for the variable domains of the heavy and light chains of human immunoglobulins. The genes were cloned from lymphocytes of four subjects vaccinated with the vaccinia virus (VACV). The library included 3 · 107 independent clones. After enrichment with clones producing scAbs against a recombinant analog of the variola virus envelope protein prA30L, the library was used to select a panel of scAbs binding both prA30L and VACV. All scAbs selected were tested for virus-neutralizing activity, and two scAbs proved to suppress VACV plaque formation in monolayers of Vero E6 cells. The specificity of antigen binding was verified by ELISA and Western blotting. The amino acid sequences of the virus-neutralizing scAbs were determined by sequencing their genes.
Citations by journals
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1
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Virus Research
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Virus Research
1 publication, 33.33%
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Molecular Biology
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Molecular Biology
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Vavilovskii Zhurnal Genetiki i Selektsii (Vavilov Journal of Genetics and Breeding)
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1 publication, 33.33%
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Citations by publishers
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1
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Elsevier
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Elsevier
1 publication, 33.33%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 33.33%
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Institute of Cytology and Genetics SB RAS
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Institute of Cytology and Genetics SB RAS
1 publication, 33.33%
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1
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Dubrovskaya V. V. et al. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.
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Dubrovskaya V. V., Ulitin A. B., Laman A. G., Gileva I., Bormotov N., Ilyichev A. A., Brovko F. A., Shchelkunov S. N., Belanov E., Tikunova N. V. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.
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TY - JOUR
DO - 10.1134/S0026893307010207
UR - https://doi.org/10.1134%2FS0026893307010207
TI - Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus
T2 - Molecular Biology
AU - Dubrovskaya, V V
AU - Ulitin, A B
AU - Laman, A G
AU - Gileva, I.P.
AU - Bormotov, N.I.
AU - Ilyichev, A. A.
AU - Brovko, F A
AU - Shchelkunov, S N
AU - Belanov, E.F.
AU - Tikunova, N V
PY - 2007
DA - 2007/02/26 00:00:00
PB - Pleiades Publishing
SP - 157-167
IS - 1
VL - 41
SN - 0026-8933
SN - 1608-3245
ER -
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@article{2007_Dubrovskaya,
author = {V V Dubrovskaya and A B Ulitin and A G Laman and I.P. Gileva and N.I. Bormotov and A. A. Ilyichev and F A Brovko and S N Shchelkunov and E.F. Belanov and N V Tikunova},
title = {Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus},
journal = {Molecular Biology},
year = {2007},
volume = {41},
publisher = {Pleiades Publishing},
month = {feb},
url = {https://doi.org/10.1134%2FS0026893307010207},
number = {1},
pages = {157--167},
doi = {10.1134/S0026893307010207}
}
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Dubrovskaya, V. V., et al. “Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus.” Molecular Biology, vol. 41, no. 1, Feb. 2007, pp. 157-167. https://doi.org/10.1134%2FS0026893307010207.
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