Molecular Biology

, том 41 , издание 1 , страницы 157-167

Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus

Тип публикации: Journal Article

Дата публикации: 2007-02-26

Pleiades Publishing

Molecular Biology

scimago Q3

wos Q4

БС3

SJR: 0.331

CiteScore: 1.6

Impact factor: 1.2

ISSN: 00268933, 16083245, 21689547

DOI: 10.1134/S0026893307010207

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Structural Biology

Biophysics

Краткое описание

A combinatorial immune library of human single-chain antibodies (scAbs) was constructed using the genes coding for the variable domains of the heavy and light chains of human immunoglobulins. The genes were cloned from lymphocytes of four subjects vaccinated with the vaccinia virus (VACV). The library included 3 · 107 independent clones. After enrichment with clones producing scAbs against a recombinant analog of the variola virus envelope protein prA30L, the library was used to select a panel of scAbs binding both prA30L and VACV. All scAbs selected were tested for virus-neutralizing activity, and two scAbs proved to suppress VACV plaque formation in monolayers of Vero E6 cells. The specificity of antigen binding was verified by ELISA and Western blotting. The amino acid sequences of the virus-neutralizing scAbs were determined by sequencing their genes.

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Журналы

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Virus Research	Virus Research, 1, 33.33% Virus Research 1 публикация, 33.33%
Molecular Biology	Molecular Biology, 1, 33.33% Molecular Biology 1 публикация, 33.33%
Vavilovskii Zhurnal Genetiki i Selektsii (Vavilov Journal of Genetics and Breeding)	Vavilovskii Zhurnal Genetiki i Selektsii (Vavilov Journal of Genetics and Breeding), 1, 33.33% Vavilovskii Zhurnal Genetiki i Selektsii (Vavilov Journal of Genetics and Breeding) 1 публикация, 33.33%
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Elsevier	Elsevier, 1, 33.33% Elsevier 1 публикация, 33.33%
Pleiades Publishing	Pleiades Publishing, 1, 33.33% Pleiades Publishing 1 публикация, 33.33%
Institute of Cytology and Genetics SB RAS	Institute of Cytology and Genetics SB RAS, 1, 33.33% Institute of Cytology and Genetics SB RAS 1 публикация, 33.33%
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Dubrovskaya V. V. et al. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.

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Dubrovskaya V. V., Ulitin A. B., Laman A. G., Gileva I., Bormotov N., Ilyichev A. A., Brovko F. A., Shchelkunov S. N., Belanov E., Tikunova N. V. Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus // Molecular Biology. 2007. Vol. 41. No. 1. pp. 157-167.

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TY - JOUR

DO - 10.1134/S0026893307010207

UR - https://doi.org/10.1134/S0026893307010207

TI - Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus

T2 - Molecular Biology

AU - Dubrovskaya, V V

AU - Ulitin, A B

AU - Laman, A G

AU - Gileva, I.P.

AU - Bormotov, N.I.

AU - Ilyichev, A. A.

AU - Brovko, F A

AU - Shchelkunov, S N

AU - Belanov, E.F.

AU - Tikunova, N V

PY - 2007

DA - 2007/02/26

PB - Pleiades Publishing

SP - 157-167

IS - 1

VL - 41

SN - 0026-8933

SN - 1608-3245

SN - 2168-9547

ER -

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@article{2007_Dubrovskaya,

author = {V V Dubrovskaya and A B Ulitin and A G Laman and I.P. Gileva and N.I. Bormotov and A. A. Ilyichev and F A Brovko and S N Shchelkunov and E.F. Belanov and N V Tikunova},

title = {Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus},

journal = {Molecular Biology},

year = {2007},

volume = {41},

publisher = {Pleiades Publishing},

month = {feb},

url = {https://doi.org/10.1134/S0026893307010207},

number = {1},

pages = {157--167},

doi = {10.1134/S0026893307010207}

}

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Dubrovskaya, V. V., et al. “Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus.” Molecular Biology, vol. 41, no. 1, Feb. 2007, pp. 157-167. https://doi.org/10.1134/S0026893307010207.