Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein
Publication type: Journal Article
Publication date: 2009-08-25
scimago Q3
wos Q4
SJR: 0.331
CiteScore: 1.6
Impact factor: 1.2
ISSN: 00268933, 16083245, 21689547
Structural Biology
Biophysics
Abstract
Single-stranded DNA aptamers interacting with fibrils of Saccharomyces cerevisiae Sup35p were obtained by the SELEX procedure. The specificity of interactions with Sup35p was investigated for 10 out of 40 selected aptamers. Nine aptamers were found to bind to the fibrillar but not to the monomeric form of Sup35p. The dissociation constant of the aptamer-fibril complex ranged from 0.1 to 1.0 μM for different aptamers. The aptamers can be used to study the prion transformation of Sup35p.
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Surina E. R. et al. Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein // Molecular Biology. 2009. Vol. 43. No. 4. pp. 626-631.
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Surina E. R., Morozkina E. V., Marchenko A., Antipin A. A., Mitkevich O. V., Kushnirov V. V., Ter-Avanesyan M. D., Benevolensky S. Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein // Molecular Biology. 2009. Vol. 43. No. 4. pp. 626-631.
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RIS
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TY - JOUR
DO - 10.1134/s0026893309040153
UR - https://doi.org/10.1134/s0026893309040153
TI - Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein
T2 - Molecular Biology
AU - Surina, E R
AU - Morozkina, E. V.
AU - Marchenko, A.N.
AU - Antipin, A A
AU - Mitkevich, O. V.
AU - Kushnirov, V V
AU - Ter-Avanesyan, M. D.
AU - Benevolensky, S.V.
PY - 2009
DA - 2009/08/25
PB - Pleiades Publishing
SP - 626-631
IS - 4
VL - 43
SN - 0026-8933
SN - 1608-3245
SN - 2168-9547
ER -
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BibTex (up to 50 authors)
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@article{2009_Surina,
author = {E R Surina and E. V. Morozkina and A.N. Marchenko and A A Antipin and O. V. Mitkevich and V V Kushnirov and M. D. Ter-Avanesyan and S.V. Benevolensky},
title = {Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein},
journal = {Molecular Biology},
year = {2009},
volume = {43},
publisher = {Pleiades Publishing},
month = {aug},
url = {https://doi.org/10.1134/s0026893309040153},
number = {4},
pages = {626--631},
doi = {10.1134/s0026893309040153}
}
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Surina, E. R., et al. “Selection of DNA aptamers specifically interacting with the fibrillar form of the yeast Sup35 protein.” Molecular Biology, vol. 43, no. 4, Aug. 2009, pp. 626-631. https://doi.org/10.1134/s0026893309040153.