The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C
V.A Tarasov
1
,
N V Boyko
1
,
M A Makhotkin
1
,
E F Shin
1
,
M G Tyutyakina
1
,
I E Chikunov
1
,
A V Naboka
1
,
A N Mashkarina
1
,
A A Kirpiy
2
,
D. G. Matishov
1
2
InterLabService Co., Ltd., Moscow, Russia
|
Publication type: Journal Article
Publication date: 2016-11-23
scimago Q4
wos Q4
SJR: 0.181
CiteScore: 1.0
Impact factor: 0.5
ISSN: 10227954, 16083369
Genetics
Abstract
The dependence of expression of miRNAs and their precursors (pre-miRNAs) on the DNA methylation level in HeLa cells 8 days after mitomycin C treatment was studied. A massive parallel DNA sequencing method was applied to analyze miRNA expression. 5-Azacytidine (DNA methylation inhibitor) was added to the medium 6 days after mutagenic agent exposure. The results indicated that the change in expression for some mature miRNAs (39 of 61) was accompanied by the change in the expression of their pre-miRNAs, while there were no significant changes in the expression of pre-miRNA for other mature miRNAs (22 of 61). The aberrant expression was maintained by 8 of 61 mature miRNAs and 6 of 55 pre-miRNAs in the induced HeLa cells after 5-azacytidine treatment. In addition, the expression of more than 90% of miRNAs, which indicated a significant change in expression after mitomycin C treatment, does not depend or depends slightly on the DNA methylation level in HeLa cells without mitomycin C treatment. The results suggest that mitomycin C induces aberrant DNA methylation which affects maintenance of changes in the miRNA expression in cell generations after mutagen treatment.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
1
2
|
|
|
Russian Journal of Genetics
2 publications, 100%
|
|
|
1
2
|
Publishers
|
1
2
|
|
|
Pleiades Publishing
2 publications, 100%
|
|
|
1
2
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
2
Total citations:
2
Citations from 2025:
0
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Tarasov V. et al. The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C // Russian Journal of Genetics. 2016. Vol. 52. No. 11. pp. 1117-1123.
GOST all authors (up to 50)
Copy
Tarasov V., Boyko N. V., Makhotkin M. A., Shin E. F., Tyutyakina M. G., Chikunov I. E., Naboka A. V., Mashkarina A. N., Kirpiy A. A., Matishov D. G. The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C // Russian Journal of Genetics. 2016. Vol. 52. No. 11. pp. 1117-1123.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1134/S1022795416110156
UR - https://doi.org/10.1134/S1022795416110156
TI - The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C
T2 - Russian Journal of Genetics
AU - Tarasov, V.A
AU - Boyko, N V
AU - Makhotkin, M A
AU - Shin, E F
AU - Tyutyakina, M G
AU - Chikunov, I E
AU - Naboka, A V
AU - Mashkarina, A N
AU - Kirpiy, A A
AU - Matishov, D. G.
PY - 2016
DA - 2016/11/23
PB - Pleiades Publishing
SP - 1117-1123
IS - 11
VL - 52
SN - 1022-7954
SN - 1608-3369
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2016_Tarasov,
author = {V.A Tarasov and N V Boyko and M A Makhotkin and E F Shin and M G Tyutyakina and I E Chikunov and A V Naboka and A N Mashkarina and A A Kirpiy and D. G. Matishov},
title = {The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C},
journal = {Russian Journal of Genetics},
year = {2016},
volume = {52},
publisher = {Pleiades Publishing},
month = {nov},
url = {https://doi.org/10.1134/S1022795416110156},
number = {11},
pages = {1117--1123},
doi = {10.1134/S1022795416110156}
}
Cite this
MLA
Copy
Tarasov, V.A, et al. “The miRNA aberrant expression dependence on DNA methylation in HeLa cells treated with mitomycin C.” Russian Journal of Genetics, vol. 52, no. 11, Nov. 2016, pp. 1117-1123. https://doi.org/10.1134/S1022795416110156.
Profiles