The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli
S V Smirnova
1
,
S. K. Abilev
1, 2
,
E V Igonina
1
,
V M Glaser
2
,
V. N. Parmon
3
,
N K Yankovsky
1, 2
Publication type: Journal Article
Publication date: 2018-08-23
scimago Q4
wos Q4
SJR: 0.181
CiteScore: 1.0
Impact factor: 0.5
ISSN: 10227954, 16083369
Genetics
Abstract
This study has shown for the first time that deuterium oxide (D2O) enhances expression of the E. coli ada-regulon induced by alkylating compounds, N-nitroso-N-methylurea (NMU) and methyl methanesulfonate (MMS). To compare the induction of ada-regulon in deuterated and in non-deuterated (control) cultures of E. coli, a biosensor based on the E. coli strain K12 MG1655 (pAlkA–lux) luminescing as a result of activating the alkA gene promoter in response to DNA alkylation was used. Depending on the D2O concentration from 5 to 10% in the pre-deuterium medium, these alkylating compounds at a concentration of 0.005 M induced expression of the alkA gene from 1.3 to 5 times higher as compared to induction in nondeuterated culture. It is suggested that deuterium can be one of the main factors in stabilizing the bond between the promoter and the alkylated Ada protein, leading to increased transcription of the ada-regulon.
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Smirnova S. V. et al. The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli // Russian Journal of Genetics. 2018. Vol. 54. No. 8. pp. 919-924.
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Smirnova S. V., Abilev S. K., Igonina E. V., Glaser V. M., Parmon V. N., Yankovsky N. K. The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli // Russian Journal of Genetics. 2018. Vol. 54. No. 8. pp. 919-924.
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RIS
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TY - JOUR
DO - 10.1134/S1022795418080124
UR - https://doi.org/10.1134/S1022795418080124
TI - The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli
T2 - Russian Journal of Genetics
AU - Smirnova, S V
AU - Abilev, S. K.
AU - Igonina, E V
AU - Glaser, V M
AU - Parmon, V. N.
AU - Yankovsky, N K
PY - 2018
DA - 2018/08/23
PB - Pleiades Publishing
SP - 919-924
IS - 8
VL - 54
SN - 1022-7954
SN - 1608-3369
ER -
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BibTex (up to 50 authors)
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@article{2018_Smirnova,
author = {S V Smirnova and S. K. Abilev and E V Igonina and V M Glaser and V. N. Parmon and N K Yankovsky},
title = {The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli},
journal = {Russian Journal of Genetics},
year = {2018},
volume = {54},
publisher = {Pleiades Publishing},
month = {aug},
url = {https://doi.org/10.1134/S1022795418080124},
number = {8},
pages = {919--924},
doi = {10.1134/S1022795418080124}
}
Cite this
MLA
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Smirnova, S. V., et al. “The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli.” Russian Journal of Genetics, vol. 54, no. 8, Aug. 2018, pp. 919-924. https://doi.org/10.1134/S1022795418080124.
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