Crystallography Reports, volume 66, issue 5, pages 854-860

Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins

Pichkur E B 1, 2
Mikirtumov V I 3
Tikhonova O V 4
Derkacheva N I 5
Kurochkina L. P. 6
Publication typeJournal Article
Publication date2021-09-01
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor0.7
ISSN10637745, 1562689X
General Chemistry
Condensed Matter Physics
General Materials Science
Abstract
Abstract The roughly purified extract of E. coli proteins has been studied by cryoelectron microscopy, the class-sums containing 2D projections of two proteins (β-galactosidase and 2-oxoglutarate dehydrogenase complex catalytic domain (ODC-CD)), identified in an extract by tandem mass spectrometry, have been distinguished. The structures of these proteins have been solved at near-atomic resolution. De novo simulation of the ODC-CD structure yielded an atomic model that revealed differences in the positions of some amino acid residues of the active center, in comparison with the known crystal structures.

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Acta Crystallographica Section D: Structural Biology
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Acta Crystallographica Section D: Structural Biology
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International Union of Crystallography (IUCr)
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International Union of Crystallography (IUCr)
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Pichkur E. B. et al. Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins // Crystallography Reports. 2021. Vol. 66. No. 5. pp. 854-860.
GOST all authors (up to 50) Copy
Pichkur E. B., Mikirtumov V. I., Tikhonova O. V., Derkacheva N. I., Kurochkina L. P., Sokolova O. S. Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins // Crystallography Reports. 2021. Vol. 66. No. 5. pp. 854-860.
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TY - JOUR
DO - 10.1134/S1063774521050187
UR - https://doi.org/10.1134%2FS1063774521050187
TI - Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins
T2 - Crystallography Reports
AU - Pichkur, E B
AU - Mikirtumov, V I
AU - Tikhonova, O V
AU - Derkacheva, N I
AU - Kurochkina, L. P.
AU - Sokolova, O. S.
PY - 2021
DA - 2021/09/01 00:00:00
PB - Pleiades Publishing
SP - 854-860
IS - 5
VL - 66
SN - 1063-7745
SN - 1562-689X
ER -
BibTex |
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BibTex Copy
@article{2021_Pichkur,
author = {E B Pichkur and V I Mikirtumov and O V Tikhonova and N I Derkacheva and L. P. Kurochkina and O. S. Sokolova},
title = {Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins},
journal = {Crystallography Reports},
year = {2021},
volume = {66},
publisher = {Pleiades Publishing},
month = {sep},
url = {https://doi.org/10.1134%2FS1063774521050187},
number = {5},
pages = {854--860},
doi = {10.1134/S1063774521050187}
}
MLA
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Pichkur, E. B., et al. “Determination of the Near-Atomic Structure of Non-Purified Oligomeric E. coli Proteins.” Crystallography Reports, vol. 66, no. 5, Sep. 2021, pp. 854-860. https://doi.org/10.1134%2FS1063774521050187.
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