Technical Physics Letters, volume 43, issue 12, pages 1088-1091
Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody
Eliseev I E
1
,
Yudenko A N
1
,
Besedina N. A.
1
,
Ulitin A B
2
,
Ekimova V M
2
,
Evdokimov S R
2
,
Putintceva J V
2
,
Yakovlev P. A.
2
,
Lomovskaya M I
2
,
Terterov I N
1
,
Bogdanov A. A.
1
,
Dubina M V
3
Publication type: Journal Article
Publication date: 2017-12-01
Journal:
Technical Physics Letters
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor: 0.6
ISSN: 10637850, 10906533, 17267471
Physics and Astronomy (miscellaneous)
Abstract
The stability of a new single-domain therapeutic antibody to the ErbB3 receptor was studied by fluorescence spectroscopy at different concentrations of a denaturing agent and temperatures. The analysis of experimental denaturation curves allowed us to build a complete thermodynamic model of unfolding and to determine all parameters of the transition: ΔG = 8.5 kcal mol–1, Tm = 76°C, ΔHm = 107 kcal mol–1, ΔCp = 1.8 kcal K–1 mol–1. The obtained data evidence the high stability of the antibody in a broad range of conditions, which is essential for further structural and functional studies and possible therapeutic application.
Citations by journals
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F1000Research
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F1000Research
2 publications, 50%
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Biomedicines
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Biomedicines
1 publication, 25%
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Pharmaceutical Research
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Pharmaceutical Research
1 publication, 25%
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Citations by publishers
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F1000 Research
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F1000 Research
2 publications, 50%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 25%
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Springer Nature
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Springer Nature
1 publication, 25%
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1
2
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- We do not take into account publications that without a DOI.
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Eliseev I. E. et al. Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody // Technical Physics Letters. 2017. Vol. 43. No. 12. pp. 1088-1091.
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Eliseev I. E., Yudenko A. N., Besedina N. A., Ulitin A. B., Ekimova V. M., Evdokimov S. R., Putintceva J. V., Yakovlev P. A., Lomovskaya M. I., Terterov I. N., Bogdanov A. A., Dubina M. V. Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody // Technical Physics Letters. 2017. Vol. 43. No. 12. pp. 1088-1091.
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TY - JOUR
DO - 10.1134/S1063785017120045
UR - https://doi.org/10.1134%2FS1063785017120045
TI - Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody
T2 - Technical Physics Letters
AU - Eliseev, I E
AU - Yudenko, A N
AU - Besedina, N. A.
AU - Ulitin, A B
AU - Ekimova, V M
AU - Evdokimov, S R
AU - Putintceva, J V
AU - Yakovlev, P. A.
AU - Lomovskaya, M I
AU - Terterov, I N
AU - Bogdanov, A. A.
AU - Dubina, M V
PY - 2017
DA - 2017/12/01 00:00:00
PB - Pleiades Publishing
SP - 1088-1091
IS - 12
VL - 43
SN - 1063-7850
SN - 1090-6533
SN - 1726-7471
ER -
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@article{2017_Eliseev,
author = {I E Eliseev and A N Yudenko and N. A. Besedina and A B Ulitin and V M Ekimova and S R Evdokimov and J V Putintceva and P. A. Yakovlev and M I Lomovskaya and I N Terterov and A. A. Bogdanov and M V Dubina},
title = {Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody},
journal = {Technical Physics Letters},
year = {2017},
volume = {43},
publisher = {Pleiades Publishing},
month = {dec},
url = {https://doi.org/10.1134%2FS1063785017120045},
number = {12},
pages = {1088--1091},
doi = {10.1134/S1063785017120045}
}
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Eliseev, I. E., et al. “Thermodynamic Analysis of the Conformational Stability of a Single-Domain Therapeutic Antibody.” Technical Physics Letters, vol. 43, no. 12, Dec. 2017, pp. 1088-1091. https://doi.org/10.1134%2FS1063785017120045.
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