Russian Journal of Bioorganic Chemistry, volume 35, issue 2, pages 176-184

Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus

Publication typeJournal Article
Publication date2009-03-30
Quartile SCImago
Q4
Quartile WOS
Q4
Impact factor1
ISSN10681620, 1608330X
Organic Chemistry
Biochemistry
Abstract
We describe here the cloning, expression, and production of specific single-chain antibodies (scFv) against the recombinant enterotoxin C1 of Staphylococcus aureus. High-affinity scFv were selected from the phage library of human mini antibodies; afterwards, the cells of E. coli trxA gor double mutant were infected with a product obtained by fusion of DNA encoding of these mini antibodies with the trxA gene to induce soluble scFv synthesis in cell cytoplasm. The scFv obtained displayed high enterotoxin C1 affinity. Analysis for cross reactivity showed that mini antibodies interacted also with SEA- SEB-, SED-, SEE-, SEG-, and SEI-type enterotoxins, but they failed to interact with ricin, diphtheritic, and cholera toxins, or with both lethal and protective factors of the anthrax toxin. This property may be helpful in screening for staphylococcus enterotoxins.
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Kalinina N. et al. Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus // Russian Journal of Bioorganic Chemistry. 2009. Vol. 35. No. 2. pp. 176-184.
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Kalinina N., Samokhvalova L. V., Ulitin A. B., Vertiev Yu. V., Nesmeyanov V. Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus // Russian Journal of Bioorganic Chemistry. 2009. Vol. 35. No. 2. pp. 176-184.
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TY - JOUR
DO - 10.1134/S1068162009020058
UR - https://doi.org/10.1134%2FS1068162009020058
TI - Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus
T2 - Russian Journal of Bioorganic Chemistry
AU - Kalinina, N.A
AU - Samokhvalova, L V
AU - Ulitin, A B
AU - Vertiev, Yu V
AU - Nesmeyanov, V.A.
PY - 2009
DA - 2009/03/30 00:00:00
PB - Pleiades Publishing
SP - 176-184
IS - 2
VL - 35
SN - 1068-1620
SN - 1608-330X
ER -
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@article{2009_Kalinina
author = {N.A Kalinina and L V Samokhvalova and A B Ulitin and Yu V Vertiev and V.A. Nesmeyanov},
title = {Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2009},
volume = {35},
publisher = {Pleiades Publishing},
month = {mar},
url = {https://doi.org/10.1134%2FS1068162009020058},
number = {2},
pages = {176--184},
doi = {10.1134/S1068162009020058}
}
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Kalinina, N.A, et al. “Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus.” Russian Journal of Bioorganic Chemistry, vol. 35, no. 2, Mar. 2009, pp. 176-184. https://doi.org/10.1134%2FS1068162009020058.
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