Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus
Publication type: Journal Article
Publication date: 2009-03-30
scimago Q4
wos Q3
SJR: 0.267
CiteScore: 1.9
Impact factor: 1.7
ISSN: 10681620, 1608330X, 15739163
PubMed ID:
19537170
Organic Chemistry
Biochemistry
Abstract
We describe here the cloning, expression, and production of specific single-chain antibodies (scFv) against the recombinant enterotoxin C1 of Staphylococcus aureus. High-affinity scFv were selected from the phage library of human mini antibodies; afterwards, the cells of E. coli trxA gor double mutant were infected with a product obtained by fusion of DNA encoding of these mini antibodies with the trxA gene to induce soluble scFv synthesis in cell cytoplasm. The scFv obtained displayed high enterotoxin C1 affinity. Analysis for cross reactivity showed that mini antibodies interacted also with SEA- SEB-, SED-, SEE-, SEG-, and SEI-type enterotoxins, but they failed to interact with ricin, diphtheritic, and cholera toxins, or with both lethal and protective factors of the anthrax toxin. This property may be helpful in screening for staphylococcus enterotoxins.
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Kalinina N. et al. Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus // Russian Journal of Bioorganic Chemistry. 2009. Vol. 35. No. 2. pp. 176-184.
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Kalinina N., Samokhvalova L. V., Ulitin A. B., Vertiev Yu. V., Nesmeyanov V. Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus // Russian Journal of Bioorganic Chemistry. 2009. Vol. 35. No. 2. pp. 176-184.
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TY - JOUR
DO - 10.1134/S1068162009020058
UR - https://doi.org/10.1134/S1068162009020058
TI - Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus
T2 - Russian Journal of Bioorganic Chemistry
AU - Kalinina, N.A
AU - Samokhvalova, L V
AU - Ulitin, A B
AU - Vertiev, Yu V
AU - Nesmeyanov, V.A.
PY - 2009
DA - 2009/03/30
PB - Pleiades Publishing
SP - 176-184
IS - 2
VL - 35
PMID - 19537170
SN - 1068-1620
SN - 1608-330X
SN - 1573-9163
ER -
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@article{2009_Kalinina,
author = {N.A Kalinina and L V Samokhvalova and A B Ulitin and Yu V Vertiev and V.A. Nesmeyanov},
title = {Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2009},
volume = {35},
publisher = {Pleiades Publishing},
month = {mar},
url = {https://doi.org/10.1134/S1068162009020058},
number = {2},
pages = {176--184},
doi = {10.1134/S1068162009020058}
}
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Kalinina, N.A, et al. “Molecular cloning, expression and characterization of human mini antibodies against enterotoxin C1 of Staphylococcus aureus.” Russian Journal of Bioorganic Chemistry, vol. 35, no. 2, Mar. 2009, pp. 176-184. https://doi.org/10.1134/S1068162009020058.
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