Pleiades Publishing
Russian Journal of Bioorganic Chemistry
,  volume 41 ,  issue 5 ,  pages 525-528

Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter

Publication type: Journal Article
Publication date: 2015-09-22
Pleiades Publishing
scimago Q4
wos Q3
SJR: 0.267
CiteScore: 1.9
Impact factor: 1.7
ISSN: 10681620, 1608330X, 15739163
Organic Chemistry
Biochemistry
Abstract
The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells—high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.
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Pereverzev A. P. et al. Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter // Russian Journal of Bioorganic Chemistry. 2015. Vol. 41. No. 5. pp. 525-528.
GOST all authors (up to 50) Copy
Pereverzev A. P., Matlashov M. E., Staroverov D. B., Lukyanov K., Gurskaya N. G. Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter // Russian Journal of Bioorganic Chemistry. 2015. Vol. 41. No. 5. pp. 525-528.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/S1068162015050118
UR - https://doi.org/10.1134/S1068162015050118
TI - Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter
T2 - Russian Journal of Bioorganic Chemistry
AU - Pereverzev, A P
AU - Matlashov, M E
AU - Staroverov, D B
AU - Lukyanov, K.A.
AU - Gurskaya, N G
PY - 2015
DA - 2015/09/22
PB - Pleiades Publishing
SP - 525-528
IS - 5
VL - 41
PMID - 26762096
SN - 1068-1620
SN - 1608-330X
SN - 1573-9163
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2015_Pereverzev,
author = {A P Pereverzev and M E Matlashov and D B Staroverov and K.A. Lukyanov and N G Gurskaya},
title = {Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2015},
volume = {41},
publisher = {Pleiades Publishing},
month = {sep},
url = {https://doi.org/10.1134/S1068162015050118},
number = {5},
pages = {525--528},
doi = {10.1134/S1068162015050118}
}
MLA
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MLA Copy
Pereverzev, A. P., et al. “Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter.” Russian Journal of Bioorganic Chemistry, vol. 41, no. 5, Sep. 2015, pp. 525-528. https://doi.org/10.1134/S1068162015050118.
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