Russian Journal of Bioorganic Chemistry, volume 47, issue 5, pages 1034-1042
Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells
Publication type: Journal Article
Publication date: 2021-09-01
Quartile SCImago
Q4
Quartile WOS
Q4
Impact factor: 1
ISSN: 10681620, 1608330X
Organic Chemistry
Biochemistry
Abstract
Gene therapy is a promising method for the treatment of various diseases by introducing therapeutic nucleic acids, for the delivery of which cationic liposomes are widely used. This work demonstrates the ability of cationic liposomes composed of a polycationic lipid 2X3 (1,26-bis(cholest-5-en-3β-yloxycarbonylamino)-7,11,16,20-tetraazahexacosane tetrahydrochloride) and helper lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) at various ratios (2.5 : 1 (L1); 2 : 1 (L2); 1.5 : 1 (L3); 1 : 1 (L4); 1 : 1.5 (L5); 1 : 2 (L6); 1 : 2.5 (L7)) to mediate the delivery of plasmid DNA (pDNA) into HEK293 cells with an efficiency higher than that of the commercial transfectant Lipofectamine 2000. We found that transfection activity of cationic liposomes depends on their composition as well as lipoplex composition (N/P ratio). Cationic liposomes were nontoxic to HEK293 cells and were more effective than Lipofectamine 2000 at the N/P ratios ≥6/1. In this case, the highest efficiency was achieved for liposomes L2. At lower N/P ratios = 1/1, 2/1, and 4/1, liposomes L1 can be used to provide targeted NA delivery into cells. Thus, for further in vivo experiments, nontoxic and small-size liposomes L1 and L2, in which the amount of lipid 2X3 was two or more times higher than that of helper lipid DOPE, were selected.
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Pharmaceutics
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Pharmaceutics
1 publication, 100%
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 100%
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Mikheev A. A. et al. Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells // Russian Journal of Bioorganic Chemistry. 2021. Vol. 47. No. 5. pp. 1034-1042.
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Mikheev A. A., Shmendel E. V., Nazarov G., Maslov M. A. Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells // Russian Journal of Bioorganic Chemistry. 2021. Vol. 47. No. 5. pp. 1034-1042.
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TY - JOUR
DO - 10.1134/S1068162021050319
UR - https://doi.org/10.1134%2FS1068162021050319
TI - Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells
T2 - Russian Journal of Bioorganic Chemistry
AU - Maslov, M A
AU - Shmendel, E V
AU - Nazarov, G.V.
AU - Mikheev, A A
PY - 2021
DA - 2021/09/01 00:00:00
PB - Pleiades Publishing
SP - 1034-1042
IS - 5
VL - 47
SN - 1068-1620
SN - 1608-330X
ER -
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@article{2021_Mikheev,
author = {M A Maslov and E V Shmendel and G.V. Nazarov and A A Mikheev},
title = {Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells},
journal = {Russian Journal of Bioorganic Chemistry},
year = {2021},
volume = {47},
publisher = {Pleiades Publishing},
month = {sep},
url = {https://doi.org/10.1134%2FS1068162021050319},
number = {5},
pages = {1034--1042},
doi = {10.1134/S1068162021050319}
}
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Mikheev, A. A., et al. “Influence of Liposome Composition on Plasmid DNA Delivery to Eukaryotic Cells.” Russian Journal of Bioorganic Chemistry, vol. 47, no. 5, Sep. 2021, pp. 1034-1042. https://doi.org/10.1134%2FS1068162021050319.
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