Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

, том 8 , издание 2 , страницы 164-167

Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

Тип публикации: Journal Article

Дата публикации: 2014-04-01

Pleiades Publishing

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

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CiteScore: 1.0

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ISSN: 19907508, 19907516

DOI: 10.1134/S1990750814020103

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Biochemistry

Clinical Biochemistry

Molecular Medicine

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Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes using a streptavidin conjugate with gold nanoparticles. Effective imaging of DNA duplexes was possible using the conjugate prepared by covalent binding. The detection limit of the model oligonucleotide of 19 bases was 20 pg.

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Яминский Игорь

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д.ф.-м.н., проф.

200 публикаций, 3 707 цитирований

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Московский государственный университет имени М.В. Ломоносова

1 цитирование

Дубровин Евгений

д.ф.-м.н.

56 публикаций, 667 цитирований, 5 рецензий

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Московский государственный университет имени М.В. Ломоносова

Научно-технологический университет «Сириус»

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Presnova G. V. et al. Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface // Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2014. Vol. 8. No. 2. pp. 164-167.

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Presnova G. V., Rubtsova M. Yu., Presnov D. E., Grigorenko V. G., Yaminsky I. V., Egorov A. Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface // Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2014. Vol. 8. No. 2. pp. 164-167.

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TY - JOUR

DO - 10.1134/S1990750814020103

UR - https://doi.org/10.1134/S1990750814020103

TI - Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

T2 - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

AU - Presnova, G V

AU - Rubtsova, M Yu

AU - Presnov, D E

AU - Grigorenko, V G

AU - Yaminsky, I. V.

AU - Egorov, A.M

PY - 2014

DA - 2014/04/01

PB - Pleiades Publishing

SP - 164-167

IS - 2

VL - 8

SN - 1990-7508

SN - 1990-7516

ER -

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@article{2014_Presnova,

author = {G V Presnova and M Yu Rubtsova and D E Presnov and V G Grigorenko and I. V. Yaminsky and A.M Egorov},

title = {Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface},

journal = {Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry},

year = {2014},

volume = {8},

publisher = {Pleiades Publishing},

month = {apr},

url = {https://doi.org/10.1134/S1990750814020103},

number = {2},

pages = {164--167},

doi = {10.1134/S1990750814020103}

}

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Presnova, G. V., et al. “Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface.” Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, vol. 8, no. 2, Apr. 2014, pp. 164-167. https://doi.org/10.1134/S1990750814020103.