volume 8 issue 2 pages 164-167

Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface

G V Presnova 1, 2
M Yu Rubtsova 1, 2
D E Presnov 3
V G Grigorenko 1, 2
A.M Egorov 1, 2
Publication typeJournal Article
Publication date2014-04-01
scimago Q4
wos Q4
SJR0.206
CiteScore1.0
Impact factor0.4
ISSN19907508, 19907516
Biochemistry
Clinical Biochemistry
Molecular Medicine
Abstract
Applicability of scanning electron microscopy (SEM) for visualization of individual acts of DNA hybridization with oligonucleotide probes has been investigated using gold nanoparticles as a label. DNA or oligonucleotides were labeled with biotin molecules, which were then detected in DNA duplexes using a streptavidin conjugate with gold nanoparticles. Effective imaging of DNA duplexes was possible using the conjugate prepared by covalent binding. The detection limit of the model oligonucleotide of 19 bases was 20 pg.
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Presnova G. V. et al. Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface // Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2014. Vol. 8. No. 2. pp. 164-167.
GOST all authors (up to 50) Copy
Presnova G. V., Rubtsova M. Yu., Presnov D. E., Grigorenko V. G., Yaminsky I. V., Egorov A. Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface // Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry. 2014. Vol. 8. No. 2. pp. 164-167.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/S1990750814020103
UR - https://doi.org/10.1134/S1990750814020103
TI - Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface
T2 - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
AU - Presnova, G V
AU - Rubtsova, M Yu
AU - Presnov, D E
AU - Grigorenko, V G
AU - Yaminsky, I. V.
AU - Egorov, A.M
PY - 2014
DA - 2014/04/01
PB - Pleiades Publishing
SP - 164-167
IS - 2
VL - 8
SN - 1990-7508
SN - 1990-7516
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2014_Presnova,
author = {G V Presnova and M Yu Rubtsova and D E Presnov and V G Grigorenko and I. V. Yaminsky and A.M Egorov},
title = {Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface},
journal = {Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry},
year = {2014},
volume = {8},
publisher = {Pleiades Publishing},
month = {apr},
url = {https://doi.org/10.1134/S1990750814020103},
number = {2},
pages = {164--167},
doi = {10.1134/S1990750814020103}
}
MLA
Cite this
MLA Copy
Presnova, G. V., et al. “Streptavidin conjugates with gold nanoparticles for visualization of single DNA interactions on the silicon surface.” Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, vol. 8, no. 2, Apr. 2014, pp. 164-167. https://doi.org/10.1134/S1990750814020103.