Pleiades Publishing
Russian Journal of Physical Chemistry B
,  volume 4 ,  issue 3 ,  pages 510-514

The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles

Publication type: Journal Article
Publication date: 2010-06-01
Pleiades Publishing
scimago Q4
SJR: 0.244
CiteScore: 2.6
Impact factor: —
ISSN: 19907931, 19907923
Physical and Theoretical Chemistry
Abstract
A procedure for using spin-label EPR spectroscopy in studies of the adsorption of macromolecular systems on magnetic nanoparticles and quantitative characterization of adsorption layers was developed. The procedure was based on measurements of the intensity of EPR signals of stable nitroxide radical labels covalently bound with macromolecules. The adsorption of spin-labeled macromolecules on the surface of nanoparticles caused broadening of EPR lines as a result of the dipole interaction of unpaired radical electrons with the magnetic moment of particles, which decreased signal intensity. Multilayer adsorption in aqueous media of biological fibrinogen molecules and synthetic polymer (polyethyleneimine) on magnetite particles with a mean diameter of 17 nm was studied. The method was shown to be applicable to the estimation of the amount of adsorbed macromolecules in multilayer coatings up to ∼40 nm thick.
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Sorokina O. N. et al. The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles // Russian Journal of Physical Chemistry B. 2010. Vol. 4. No. 3. pp. 510-514.
GOST all authors (up to 50) Copy
Sorokina O. N., Bychkova A., Shapiro A. B., Tikhonov A. P., Kovarskii A. L. The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles // Russian Journal of Physical Chemistry B. 2010. Vol. 4. No. 3. pp. 510-514.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/S199079311003022X
UR - https://doi.org/10.1134/S199079311003022X
TI - The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles
T2 - Russian Journal of Physical Chemistry B
AU - Sorokina, O N
AU - Bychkova, A.V.
AU - Shapiro, A. B.
AU - Tikhonov, A P
AU - Kovarskii, A L
PY - 2010
DA - 2010/06/01
PB - Pleiades Publishing
SP - 510-514
IS - 3
VL - 4
SN - 1990-7931
SN - 1990-7923
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2010_Sorokina,
author = {O N Sorokina and A.V. Bychkova and A. B. Shapiro and A P Tikhonov and A L Kovarskii},
title = {The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles},
journal = {Russian Journal of Physical Chemistry B},
year = {2010},
volume = {4},
publisher = {Pleiades Publishing},
month = {jun},
url = {https://doi.org/10.1134/S199079311003022X},
number = {3},
pages = {510--514},
doi = {10.1134/S199079311003022X}
}
MLA
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MLA Copy
Sorokina, O. N., et al. “The application of the spin label method for studying the adsorption of macromolecules on magnetic nanoparticles.” Russian Journal of Physical Chemistry B, vol. 4, no. 3, Jun. 2010, pp. 510-514. https://doi.org/10.1134/S199079311003022X.