Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography
Publication type: Journal Article
Publication date: 2015-06-23
scimago Q3
wos Q4
SJR: 0.252
CiteScore: 1.9
Impact factor: 1.1
ISSN: 10619348, 16083199
Analytical Chemistry
Abstract
The stability and behavior of beet betacyanins in reversed-phase HPLC and in solid-phase purification on Diapak C18 concentrating cartridges have been studied. It has been demonstrated that, in acidic mobile phases and solutions with pH < 4.0, the decyclization of betacyanins is the main reason for their instability. The differences in the adsorption of betanin and isobetanin with changes in the pH of the mobile phase have been determined. A method has been developed for the solid-phase purification of betacyanins from natural extracts with the yield higher than 90% followed by determination by HPLC with gradient elution.
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Saenko I. I., Deineka V. I., Deineka L. A. Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography // Journal of Analytical Chemistry. 2015. Vol. 70. No. 7. pp. 892-896.
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Saenko I. I., Deineka V. I., Deineka L. A. Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography // Journal of Analytical Chemistry. 2015. Vol. 70. No. 7. pp. 892-896.
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RIS
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TY - JOUR
DO - 10.1134/s1061934815070138
UR - https://doi.org/10.1134/s1061934815070138
TI - Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography
T2 - Journal of Analytical Chemistry
AU - Saenko, I I
AU - Deineka, V I
AU - Deineka, L A
PY - 2015
DA - 2015/06/23
PB - Pleiades Publishing
SP - 892-896
IS - 7
VL - 70
SN - 1061-9348
SN - 1608-3199
ER -
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BibTex (up to 50 authors)
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@article{2015_Saenko,
author = {I I Saenko and V I Deineka and L A Deineka},
title = {Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography},
journal = {Journal of Analytical Chemistry},
year = {2015},
volume = {70},
publisher = {Pleiades Publishing},
month = {jun},
url = {https://doi.org/10.1134/s1061934815070138},
number = {7},
pages = {892--896},
doi = {10.1134/s1061934815070138}
}
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MLA
Copy
Saenko, I. I., et al. “Specific features of the determination of betacyanins by reversed-phase high-performance liquid chromatography.” Journal of Analytical Chemistry, vol. 70, no. 7, Jun. 2015, pp. 892-896. https://doi.org/10.1134/s1061934815070138.