volume 79 issue 7 pages 860-867

Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations

Publication typeJournal Article
Publication date2024-07-23
scimago Q3
wos Q4
SJR0.252
CiteScore1.9
Impact factor1.1
ISSN10619348, 16083199
Abstract
A sandwich-type voltammetric immunosensor based on conjugates of gold nanoparticles (AuNP) with specific immunoglobulins (IgG) against ovalbumin (IgG@AuNP) is developed for the determination of ovalbumin in some immunobiological preparations. The IgG@AuNP conjugate is synthesized by passive adsorption. A carbon-containing planar printed electrode modified by laser reduced graphene oxide is used as a substrate electrode for immobilizing the receptor layer of specific immunoglobulins. A possibility of the catalytic reduction of silver nitrate with a mixture of reducing agents, citric acid and metol, is considered in order to enhance the signal of AuNP. Conditions for the voltammetric recording of the electrochemical signal of silver on an immunosensor, which has been successfully tested in determining ovalbumin residues in some immunobiological drugs, are selected. Enzyme-linked immunosorbent assay is used as a reference method.
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Dorozhko E. V. et al. Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations // Journal of Analytical Chemistry. 2024. Vol. 79. No. 7. pp. 860-867.
GOST all authors (up to 50) Copy
Dorozhko E. V., Solomonenko A. N., Saqib M., Semin V. O. Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations // Journal of Analytical Chemistry. 2024. Vol. 79. No. 7. pp. 860-867.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/s1061934824700230
UR - https://link.springer.com/10.1134/S1061934824700230
TI - Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations
T2 - Journal of Analytical Chemistry
AU - Dorozhko, E V
AU - Solomonenko, A. N.
AU - Saqib, M.
AU - Semin, V O
PY - 2024
DA - 2024/07/23
PB - Pleiades Publishing
SP - 860-867
IS - 7
VL - 79
SN - 1061-9348
SN - 1608-3199
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Dorozhko,
author = {E V Dorozhko and A. N. Solomonenko and M. Saqib and V O Semin},
title = {Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations},
journal = {Journal of Analytical Chemistry},
year = {2024},
volume = {79},
publisher = {Pleiades Publishing},
month = {jul},
url = {https://link.springer.com/10.1134/S1061934824700230},
number = {7},
pages = {860--867},
doi = {10.1134/s1061934824700230}
}
MLA
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MLA Copy
Dorozhko, E. V., et al. “Electrochemical Immunosensors Based on Gold Nanoparticles for the Determination of Ovalbumin in Immunobiological Preparations.” Journal of Analytical Chemistry, vol. 79, no. 7, Jul. 2024, pp. 860-867. https://link.springer.com/10.1134/S1061934824700230.