Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering
A S Boikova
1, 2
,
Yu A Dyakova
1, 2
,
K B Ilina
1, 2
,
P.V. Konarev
1, 2
,
A E Kryukova
1, 2
,
M A Marchenkova
1, 2
,
Yu.V. Pisarevskii
1, 2
,
M. V. Kovalchuk
1, 2, 3
1
Publication type: Journal Article
Publication date: 2018-11-01
scimago Q4
wos Q4
SJR: 0.145
CiteScore: 0.8
Impact factor: 0.5
ISSN: 10637745, 1562689X
General Chemistry
Condensed Matter Physics
General Materials Science
Abstract
Proteinase K solutions have been investigated by small-angle X-ray scattering in the temperature range of 10–30°C under conditions of tetragonal crystal growth. Addition of ammonium sulfate or sodium nitrate as precipitants is shown to induce the formation of a significant fraction of protein dimers in solution (the dimer content in solution increases only slightly with a decrease in temperature). The structures of possible dimers, which form the pre-crystallization phase in solution (which is necessary for the growth of proteinase K crystals), have been investigated by analyzing the known crystal structure.
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15
Total citations:
15
Citations from 2025:
1
(6.67%)
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Boikova A. S. et al. Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering // Crystallography Reports. 2018. Vol. 63. No. 6. pp. 865-870.
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Boikova A. S., Dyakova Yu. A., Ilina K. B., Konarev P., Kryukova A. E., Marchenkova M. A., Pisarevskii Y., Kovalchuk M. V. Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering // Crystallography Reports. 2018. Vol. 63. No. 6. pp. 865-870.
Cite this
RIS
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TY - JOUR
DO - 10.1134/s1063774518060068
UR - https://doi.org/10.1134/s1063774518060068
TI - Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering
T2 - Crystallography Reports
AU - Boikova, A S
AU - Dyakova, Yu A
AU - Ilina, K B
AU - Konarev, P.V.
AU - Kryukova, A E
AU - Marchenkova, M A
AU - Pisarevskii, Yu.V.
AU - Kovalchuk, M. V.
PY - 2018
DA - 2018/11/01
PB - Pleiades Publishing
SP - 865-870
IS - 6
VL - 63
SN - 1063-7745
SN - 1562-689X
ER -
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BibTex (up to 50 authors)
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@article{2018_Boikova,
author = {A S Boikova and Yu A Dyakova and K B Ilina and P.V. Konarev and A E Kryukova and M A Marchenkova and Yu.V. Pisarevskii and M. V. Kovalchuk},
title = {Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering},
journal = {Crystallography Reports},
year = {2018},
volume = {63},
publisher = {Pleiades Publishing},
month = {nov},
url = {https://doi.org/10.1134/s1063774518060068},
number = {6},
pages = {865--870},
doi = {10.1134/s1063774518060068}
}
Cite this
MLA
Copy
Boikova, A. S., et al. “Investigation of the Pre-crystallization Stage of Proteinase K in Solution (Influence of Temperature and Precipitant Type) by Small-Angle X-Ray Scattering.” Crystallography Reports, vol. 63, no. 6, Nov. 2018, pp. 865-870. https://doi.org/10.1134/s1063774518060068.