Pleiades Publishing
Crystallography Reports
,  volume 68 ,  issue 1 ,  pages 52-56

Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus

V I Timofeev 1, 2
, 
Yu A Abramchik 1, 3
, 
N. E. Zhukhlistova 1
, 
O O Mikheeva 3
, 
M. B. Shevtsov 4
, 
E A Zayats 3
, 
D D Lykoshin 3
, 
M A Kostromina 3
, 
R S Esipov 3
, 
I. P. Kuranova 1, 2
1
 
2
 
3
 
4
 
Publication type: Journal Article
Publication date: 2023-02-01
Pleiades Publishing
scimago Q4
wos Q4
SJR: 0.145
CiteScore: 0.8
Impact factor: 0.5
ISSN: 10637745, 1562689X
General Chemistry
Condensed Matter Physics
General Materials Science
Abstract
The high-producing strain С3029/pGro7/pERDerp3 for the house dust mite allergen Der p 3 from Dermatophagoides pteronyssinus, expressing the recombinant protein in Escherichia coli in the soluble form, was constructed. A procedure was developed for the purification of the recombinant allergen. Crystals of the recombinant protein Der p 3 suitable for X-ray diffraction analysis were grown by the vapor-diffusion method. The X-ray diffraction data set was collected to 2.25 Å resolution at the European Synchrotron Radiation Facility (ESRF, France, ID23-1 beamline) at 100 K. The crystals belong to sp. gr. С121 and contain two enzyme molecules per asymmetric unit.
Found 
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Timofeev V. I. et al. Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus // Crystallography Reports. 2023. Vol. 68. No. 1. pp. 52-56.
GOST all authors (up to 50) Copy
Timofeev V. I., Abramchik Yu. A., Zhukhlistova N. E., Mikheeva O. O., Shevtsov M. B., Zayats E. A., Lykoshin D. D., Kostromina M. A., Esipov R. S., Kuranova I. P. Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus // Crystallography Reports. 2023. Vol. 68. No. 1. pp. 52-56.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1134/s106377452206027x
UR - https://doi.org/10.1134/s106377452206027x
TI - Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus
T2 - Crystallography Reports
AU - Timofeev, V I
AU - Abramchik, Yu A
AU - Zhukhlistova, N. E.
AU - Mikheeva, O O
AU - Shevtsov, M. B.
AU - Zayats, E A
AU - Lykoshin, D D
AU - Kostromina, M A
AU - Esipov, R S
AU - Kuranova, I. P.
PY - 2023
DA - 2023/02/01
PB - Pleiades Publishing
SP - 52-56
IS - 1
VL - 68
SN - 1063-7745
SN - 1562-689X
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2023_Timofeev,
author = {V I Timofeev and Yu A Abramchik and N. E. Zhukhlistova and O O Mikheeva and M. B. Shevtsov and E A Zayats and D D Lykoshin and M A Kostromina and R S Esipov and I. P. Kuranova},
title = {Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus},
journal = {Crystallography Reports},
year = {2023},
volume = {68},
publisher = {Pleiades Publishing},
month = {feb},
url = {https://doi.org/10.1134/s106377452206027x},
number = {1},
pages = {52--56},
doi = {10.1134/s106377452206027x}
}
MLA
Cite this
MLA Copy
Timofeev, V. I., et al. “Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus.” Crystallography Reports, vol. 68, no. 1, Feb. 2023, pp. 52-56. https://doi.org/10.1134/s106377452206027x.