Annual Review of Pharmacology and Toxicology, volume 55, issue 1, pages 489-511

Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy

Publication typeJournal Article
Publication date2015-01-06
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor12.5
ISSN03621642, 15454304
Pharmacology
Toxicology
Abstract
Designed ankyrin repeat proteins (DARPins) can recognize targets with specificities and affinities that equal or surpass those of antibodies, but because of their robustness and extreme stability, they allow a multitude of more advanced formats and applications. This review highlights recent advances in DARPin design, illustrates their properties, and gives some examples of their use. In research, they have been established as intracellular, real-time sensors of protein conformations and as crystallization chaperones. For future therapies, DARPins have been developed by advanced, structure-based protein engineering to selectively induce apoptosis in tumors by uncoupling surface receptors from their signaling cascades. They have also been used successfully for retargeting viruses. In ongoing clinical trials, DARPins have shown good safety and efficacy in macular degeneration diseases. These developments all ultimately exploit the high stability, solubility, and aggregation resistance of these molecules, permitting a wide range of conjugates and fusions to be produced and purified.

Top-30

Citations by journals

2
4
6
8
10
12
14
16
International Journal of Molecular Sciences
15 publications, 3.26%
Scientific Reports
15 publications, 3.26%
Nature Communications
14 publications, 3.04%
Proceedings of the National Academy of Sciences of the United States of America
10 publications, 2.17%
Cancers
9 publications, 1.96%
Journal of Molecular Biology
9 publications, 1.96%
Methods in Molecular Biology
8 publications, 1.74%
Nature Chemical Biology
7 publications, 1.52%
Journal of Biological Chemistry
7 publications, 1.52%
Protein Engineering, Design and Selection
6 publications, 1.3%
ACS Synthetic Biology
5 publications, 1.09%
PLoS ONE
4 publications, 0.87%
Antibodies
4 publications, 0.87%
Pharmaceutics
4 publications, 0.87%
Frontiers in Immunology
4 publications, 0.87%
Structure
4 publications, 0.87%
Current Opinion in Structural Biology
4 publications, 0.87%
Protein Science
4 publications, 0.87%
Angewandte Chemie
4 publications, 0.87%
Angewandte Chemie - International Edition
4 publications, 0.87%
Molecular Pharmaceutics
4 publications, 0.87%
Molecular Biology
4 publications, 0.87%
mAbs
4 publications, 0.87%
Acta Naturae
4 publications, 0.87%
Frontiers in Molecular Biosciences
3 publications, 0.65%
Nature Reviews Drug Discovery
3 publications, 0.65%
Nature Structural and Molecular Biology
3 publications, 0.65%
Drug Discovery Today
3 publications, 0.65%
Methods
3 publications, 0.65%
2
4
6
8
10
12
14
16

Citations by publishers

10
20
30
40
50
60
70
80
90
Elsevier
88 publications, 19.13%
Springer Nature
85 publications, 18.48%
Multidisciplinary Digital Publishing Institute (MDPI)
46 publications, 10%
Wiley
35 publications, 7.61%
American Chemical Society (ACS)
29 publications, 6.3%
Cold Spring Harbor Laboratory
23 publications, 5%
Taylor & Francis
20 publications, 4.35%
Frontiers Media S.A.
11 publications, 2.39%
Oxford University Press
10 publications, 2.17%
Proceedings of the National Academy of Sciences (PNAS)
10 publications, 2.17%
Public Library of Science (PLoS)
7 publications, 1.52%
American Society for Biochemistry and Molecular Biology
7 publications, 1.52%
Royal Society of Chemistry (RSC)
7 publications, 1.52%
Pleiades Publishing
4 publications, 0.87%
Acta Naturae Ltd
4 publications, 0.87%
American Association for the Advancement of Science (AAAS)
4 publications, 0.87%
The Company of Biologists
3 publications, 0.65%
International Union of Crystallography (IUCr)
3 publications, 0.65%
Mary Ann Liebert
3 publications, 0.65%
Wolters Kluwer Health
3 publications, 0.65%
Walter de Gruyter
3 publications, 0.65%
eLife Sciences Publications
3 publications, 0.65%
Bentham Science
2 publications, 0.43%
Future Medicine
2 publications, 0.43%
Portland Press
2 publications, 0.43%
Spandidos Publications
2 publications, 0.43%
Dove Medical Press
2 publications, 0.43%
American Association for Cancer Research (AACR)
2 publications, 0.43%
Rockefeller University Press
2 publications, 0.43%
10
20
30
40
50
60
70
80
90
  • We do not take into account publications without a DOI.
  • Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
  • Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
Share
Cite this
GOST |
Cite this
GOST Copy
Plückthun A. Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy // Annual Review of Pharmacology and Toxicology. 2015. Vol. 55. No. 1. pp. 489-511.
GOST all authors (up to 50) Copy
Plückthun A. Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy // Annual Review of Pharmacology and Toxicology. 2015. Vol. 55. No. 1. pp. 489-511.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1146/annurev-pharmtox-010611-134654
UR - https://doi.org/10.1146/annurev-pharmtox-010611-134654
TI - Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy
T2 - Annual Review of Pharmacology and Toxicology
AU - Plückthun, Andreas
PY - 2015
DA - 2015/01/06 00:00:00
PB - Annual Reviews
SP - 489-511
IS - 1
VL - 55
SN - 0362-1642
SN - 1545-4304
ER -
BibTex |
Cite this
BibTex Copy
@article{2015_Plückthun,
author = {Andreas Plückthun},
title = {Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy},
journal = {Annual Review of Pharmacology and Toxicology},
year = {2015},
volume = {55},
publisher = {Annual Reviews},
month = {jan},
url = {https://doi.org/10.1146/annurev-pharmtox-010611-134654},
number = {1},
pages = {489--511},
doi = {10.1146/annurev-pharmtox-010611-134654}
}
MLA
Cite this
MLA Copy
Plückthun, Andreas. “Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy.” Annual Review of Pharmacology and Toxicology, vol. 55, no. 1, Jan. 2015, pp. 489-511. https://doi.org/10.1146/annurev-pharmtox-010611-134654.
Found error?