Cartilage

, том 13 , издание 1 , страницы 194760352210759

Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates

Denis Baranovskii ^{1, 2, 3, 4}

Jan Demner ²

Sylvia Nürnberger ^{5, 6}

Alexey Lyundup ^{4, 7}

H. Redl ⁶

Heinz Redl ⁶

Morgane Hilpert ²

Sebastien Pigeot ²

Michael Krasheninnikov ⁴

Olga Krasilnikova ^{3, 7}

Ilya Klabukov ^{3, 7}

Vladimir Parshin ⁸

I Martin ²

Didier Lardinois ¹

A Barbero ²

Скрыть аффилиации авторов Показать аффилиации авторов: 8 аффилиаций

Thoracic Surgery, University Hospital Basel, Basel, Switzerland |

Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland |

Department of regenerative technologies and biofabrication, National Medical Research Radiological Center, Obninsk, Russia |

⁴

Research and Educational Resource Center for Cellular Technologies, Peoples’ Friendship University of Russia, Moscow, Russia |

⁵

Division of Trauma Surgery, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Vienna, Austria |

⁶

Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA research center, Vienna, Austria |

⁷

Department of Advanced Cell Technologies, Sechenov University, Moscow, Russia |

⁸

Institute of Clinical Medicine, Sechenov University, Moscow, Russia |

Тип публикации: Journal Article

Дата публикации: 2022-01-01

SAGE

Cartilage

scimago Q1

wos Q2

БС1

SJR: 1.064

CiteScore: 9.0

Impact factor: 2.7

ISSN: 19476035, 19476043

DOI: 10.1177/19476035221075951

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PubMed ID: 35189712

Biomedical Engineering

Immunology and Allergy

Physical Therapy, Sports Therapy and Rehabilitation

Краткое описание

Objective

Implantation of tissue-engineered tracheal grafts represents a visionary strategy for the reconstruction of tracheal wall defects after resections and may develop into a last chance for a number of patients with severe cicatricial stenosis. The use of a decellularized tracheal substrate would offer an ideally stiff graft, but the matrix density would challenge efficient remodeling into a living cartilage. In this study, we hypothesized that the pores of decellularized laser-perforated tracheal cartilage (LPTC) tissues can be colonized by adult nasal chondrocytes (NCs) to produce new cartilage tissue suitable for the repair of tracheal defects.

Design

Human, native tracheal specimens, isolated from cadaveric donors, were exposed to decellularized and laser engraving–controlled superficial perforation (300 μm depth). Human or rabbit NCs were cultured on the LPTCs for 1 week. The resulting revitalized tissues were implanted ectopically in nude mice or orthotopically in tracheal wall defects in rabbits. Tissues were assayed histologically and by microtomography analyses before and after implantation.

Results

NCs were able to efficiently colonize the pores of the LPTCs. The extent of colonization (i.e., percentage of viable cells spanning >300 μm of tissue depth), cell morphology, and cartilage matrix deposition improved once the revitalized constructs were implanted ectopically in nude mice. LPTCs could be successfully grafted onto the tracheal wall of rabbits without any evidence of dislocation or tracheal stenosis, 8 weeks after implantation. Rabbit NCs, within the LPTCs, actively produced new cartilage matrix.

Conclusion

Implantation of NC-revitalized LPTCs represents a feasible strategy for the repair of tracheal wall defects.

Найдено

5 цитирований

Клабуков Илья

🥼 🤝

к.б.н.

100 публикаций, 620 цитирований, 1 775 рецензий

Индекс Хирша: 15

Национальный медицинский исследовательский центр радиологии Минздрава России

Национальный исследовательский ядерный университет «МИФИ»

Российский Университет Дружбы Народов

Российская академия народного хозяйства и государственной службы при Президенте Российской Федерации

Области научных интересов

CAR-T-клетки

COVID-19

Electrospinning

Mesenchymal stromal cells

Биопринтинг

Воспаление

Иммунотерапия

Интерактомика протеоформ

Онкоматрикс (Oncomatrix)

Персонализированная медицина

Рак

Регенеративная медицина

Старение

Тканевая инженерия

Функционально градиентные скаффолды (Functionally graded scaffolds)

2 цитирования

Сосин Дмитрий

к.б.н.

28 публикаций, 360 цитирований

Индекс Хирша: 12

Центр стратегического планирования и управления медико-биологическими рисками здоровью ФМБА России

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Baranovskii D. et al. Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates // Cartilage. 2022. Vol. 13. No. 1. p. 194760352210759.

ГОСТ со всеми авторами (до 50) Скопировать

Baranovskii D., Demner J., Nürnberger S., Lyundup A., Redl H., Redl H., Hilpert M., Pigeot S., Krasheninnikov M., Krasilnikova O., Klabukov I., Parshin V., Martin I., Lardinois D., Barbero A. Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates // Cartilage. 2022. Vol. 13. No. 1. p. 194760352210759.

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TY - JOUR

DO - 10.1177/19476035221075951

UR - https://journals.sagepub.com/doi/10.1177/19476035221075951

TI - Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates

T2 - Cartilage

AU - Baranovskii, Denis

AU - Demner, Jan

AU - Nürnberger, Sylvia

AU - Lyundup, Alexey

AU - Redl, H.

AU - Redl, Heinz

AU - Hilpert, Morgane

AU - Pigeot, Sebastien

AU - Krasheninnikov, Michael

AU - Krasilnikova, Olga

AU - Klabukov, Ilya

AU - Parshin, Vladimir

AU - Martin, I

AU - Lardinois, Didier

AU - Barbero, A

PY - 2022

DA - 2022/01/01

PB - SAGE

SP - 194760352210759

IS - 1

VL - 13

PMID - 35189712

SN - 1947-6035

SN - 1947-6043

ER -

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@article{2022_Baranovskii,

author = {Denis Baranovskii and Jan Demner and Sylvia Nürnberger and Alexey Lyundup and H. Redl and Heinz Redl and Morgane Hilpert and Sebastien Pigeot and Michael Krasheninnikov and Olga Krasilnikova and Ilya Klabukov and Vladimir Parshin and I Martin and Didier Lardinois and A Barbero},

title = {Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates},

journal = {Cartilage},

year = {2022},

volume = {13},

publisher = {SAGE},

month = {jan},

url = {https://journals.sagepub.com/doi/10.1177/19476035221075951},

number = {1},

pages = {194760352210759},

doi = {10.1177/19476035221075951}

}

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Baranovskii, Denis, et al. “Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates.” Cartilage, vol. 13, no. 1, Jan. 2022, p. 194760352210759. https://journals.sagepub.com/doi/10.1177/19476035221075951.